Investing the impact of Cineole on Melanoma

Abstract

Pharmaceutical ingredients have utilized naturally occurring small molecules of plants medicinally (2). 1,8-Cineole, also known as Eucalyptol, is a naturally derived compound found in eucalyptus, rosemary, and camphor laurel. 1,8-Cineole’s effect on melanoma and non-melanoma skin cancers and colon cancer exhibits inhibitory properties (4 and 5). Eucalyptol acts as an AhR inhibitor and delays tumor incidence and tumor number in UVB-induced SKH-1 mice while inhibiting COX-2 expression when applied topically (3 and 5). The anti-tumor mechanisms of eucalyptol are exhibited through the negative effect on the positive regulation of the cell cycle by reducing the expression of most genes in transcriptome sequencing and bioinformatic analysis with human neuroblastoma cells. This indicates that eucalyptol intervenes in cancer cell growth by inducing apoptosis and anti-proliferation (1). Our preliminary studies showed that 1,8-Cineole acts as a tumor suppressor in A375P cells using the migration of assay. Migration rate decreased after transfection in both 10 mM and 30 mM 1,8-Cineole 24, 48h, and 72h post scratch (P-values for 10 mM 0.29,0.004, 0.04 accordingly and 0.0001, 0.0001, and 0.002 for 30 mM, accordingly). 1. Gao K, Wu C, Li Y, Lu J, Jiang Y. Transcriptome Analysis Reveals the Anti-Tumor Mechanism of Eucalyptol Treatment on Neuroblastoma Cell Line SH-SY5Y. Neurochem Res. 2022 Dec;47(12):3854-3862. doi: 10.1007/s11064-022-03786-8. Epub 2022 Nov 4. Erratum in: Neurochem Res. 2022 Dec;47(12):3863. doi: 10.1007/s11064-022-03823-6. PMID: 36331666; PMCID: PMC9718713. 2. Hoch CC, Petry J, Griesbaum L, Weiser T, Werner K, Ploch M, Verschoor A, Multhoff G, Bashiri Dezfouli A, Wollenberg B. 1,8-cineole (eucalyptol): A versatile phytochemical with therapeutic applications across multiple diseases. Biomed Pharmacother. 2023 Nov;167:115467. doi: 10.1016/j.biopha.2023.115467. Epub 2023 Sep 9. PMID: 37696087. 3. Lee J, Ha SJ, Park J, Kim YH, Lee NH, Kim YE, Kim Y, Song KM, Jung SK. 1,8-cineole prevents UVB-induced skin carcinogenesis by targeting the aryl hydrocarbon receptor. Oncotarget. 2017 Nov 20;8(62):105995-106008. doi: 10.18632/oncotarget.22519. PMID: 29285309; PMCID: PMC5739696. 4. Murata S, Shiragami R, Kosugi C, Tezuka T, Yamazaki M, Hirano A, Yoshimura Y, Suzuki M, Shuto K, Ohkohchi N, Koda K. Antitumor effect of 1, 8-cineole against colon cancer. Oncol Rep. 2013 Dec;30(6):2647-52. doi: 10.3892/or.2013.2763. Epub 2013 Oct 1. PMID: 24085263. 5. Yoon YE, Jung YJ, Lee SJ. The Anticancer Activities of Natural Terpenoids That Inhibit Both Melanoma and Non-Melanoma Skin Cancers. Int J Mol Sci. 2024 Apr 17;25(8):4423. doi: 10.3390/ijms25084423. PMID: 38674007; PMCID: PMC11050645