Monitoring method for uranium concentration and chemical form in the droplet of rat serum

Abstract

A simple monitoring method has been proposed by measuring uranium (U) concentration and its chemical form in serum. The droplet of the 1 μL serum specimen was then examined by subjecting it to high energy synchrotron radiation X-ray fluorescence spectroscopy (SR-XRF) and X-ray absorption fine structure (XAFS) to determine the concentration and chemical form of U. The detection limit of U in 1 μL droplet was calculated to be 0.071 μg/g. The U concentration in the specimen obtained from the rat exposed to U was consistent with that determined by inductiveluy coupled plasma mass spectrometry. Uranium in the serum was estimated to be hexavalent U based on the standard specimens of tetravalent and hexavanet U. This method developed is expected to be used for monitoring and decorporation of patients at nuclear disasters and environmental pollution.journal articl