The objective of this work was to evaluate the antagonistic potential of endophytic bacteria isolated from \u27Cereja Vermelho\u27, \u27Rasteiro Rio Grande\u27, and \u27Chapolin\u27 tomato seedlings against the ALT23 Alternaria sp. fungus. Of the 23 endophytic isolates obtained, 12 were selected due to their greater effect on the mycelial growth of ALT23. Antagonism was tested in a completely randomized design, with 13 treatments (ALT23 and 12 isolates), with four replicates. The selected endophytic bacteria inhibited from 29.8 to 64.2% of the mycelial diameter and from 33.7 to 72.4% of the growth rate of ALT23.O objetivo deste trabalho foi avaliar o potencial antagônico de bactérias endofíticas isoladas de mudas de tomate \u27Cereja Vermelho\u27, \u27Rasteiro Rio Grande\u27 e \u27Chapolin\u27 contra o fungo Alternaria sp. ALT23. Dos 23 isolados endofíticos obtidos, 12 foram selecionados por terem apresentado maior efeito sobre o crescimento micelial do ALT23. O antagonismo foi testado em delineamento inteiramente casualizado, com 13 tratamentos (ALT23 e 12 isolados), com quatro repetições. As bactérias endofíticas selecionadas inibiram de 29,8 a 64,2% o diâmetro micelial e de 33,7 a 72,4% a velocidade de crescimento do ALT23
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