Biological, Biochemical and Elemental Traits of Clavelina oblonga, an Invasive Tunicate in the Adriatic Sea

Abstract

Clavelina oblonga is an invasive tropical tunicate recently introduced into the Adriatic Sea as a consequence of globalization and climate change. Mussel aquaculture sites provide an ideal environment for this colonial ascidian, where it has recently become the dominant fouling species. This study represents the first investigation of its biological and physical characteristics, as well as its proximal, fatty acid, macroelement, trace element, and toxic metal composition. The entire-tissue chemical composition of C. oblonga resulted in 95.44% moisture. Its composite structure revealed several strong peaks, attributed to O-H, C-H, C-N, and C=O stretching, along with cellulose components overlapping with proteins and carbohydrates. The major fatty acids were palmitic, stearic, and docosahexaenoic acid, followed by docosanoic, elaidic, linoleic, and myristic acid. The saturated fatty acids, polyunsaturated fatty acids, and monounsaturated fatty acids comprised 51.37, 26.96, and 15.41% of the total fatty acids, respectively. Among the analysed trace and macroelements, aluminium and sodium were predominant. C. oblonga exhibited different concentrations of toxic metals, such as arsenic and lead, compared to fouled mussels in the Istria region. It appears that the tunicate has adapted to the environmental conditions of the Adriatic, reaching its maximum spread and biomass in mid-autumn. There is a strong possibility that C. oblonga could colonize and establish itself permanently in the Adriatic. This would have a strong negative impact on shellfish farming, the structure of the ecosystem, plankton biomass, and the distribution of other marine species. However, it also represents a biomass resource with high potential of utilization in different industries