Listeria monocytogenes is ubiquitous and causes listeriosis, which manifests in animals in the form of septicaemia, encephalitis, abortions, and, rarely, mastitis. In humans, food is the main source of infection, which is the reason food business operators take samples and test for Listeria. We report a case of L. monocytogenesdetection in traditional cheese made from raw milk, and subsequent investigation revealed that L. monocytogenes was excreted from a cow mammary gland. Although treatment was carried out according to the results of the antibiogram, there was no improvement in the subclinical mastitis and the excretion of L. monocytogenes after treatment was 8.8x102cfu/mL, and therefore the cow was removed from the herd
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