The Effects of L-Theanine on Memory, Locomotion, and Anxiety in Drosophila melanogaster

Abstract

Homeopathic supplements have been marketed as therapeutic agents that promote a variety of health benefits. Although homeopathy continues to be practiced as a form of medicine, the effectiveness and safety measures of this approach have not been thoroughly studied. Previous research has proposed the use of L-Theanine as a method for improving neuroprotective functions, alleviating anxiety, and strengthening cognitive abilities, but the scope of the physiological effects mediated by L-Theanine remains unclear. Supporting or refuting the claims associated with L-Theanine through experimental research on model organisms could lead to improvements of current research on the supplement and its potential use in alternative health treatments. Based on prior findings, it was hypothesized that the addition of L-Theanine to the diet of Drosophila melanogaster would increase expression of the Gad1 and per genes, which play a role in neuronal inhibition and circadian rhythm regulation, respectively. It was further hypothesized that an upregulation of these genes would be supported by enhanced memory, slower locomotion, and reduced anxiety. To test these hypotheses, RT-qPCR analysis was performed to assess changes in gene expression and behavioral assays were conducted on visual memory, wall-climbing, and wall-following. The results from this study showed no significant changes in expression of the Gad1 and per genes between the control and L-Theanine-treated groups of D. melanogaster. Additionally, no significant changes in visual memory, locomotion, and anxiety-related behavior were found across both groups, indicating a lack of influence of L-Theanine on nervous system function