Testing the contribution of vertebrate predators and leaf traits to mainland-island differences in insect herbivory on oaks

Abstract

Ecological theory predicts that herbivory should be weaker on islands than on mainland based on the assumption that islands have lower herbivore abundance and diversity. However, empirical tests of this prediction are rare, especially for insect herbivores, and those few tests often fail to address the mechanisms behind island–mainland divergence in herbivory. In particular, past studies have not addressed the relative contribution of top-down (i.e. predator-driven) and bottom-up (i.e. plant-driven) factors to these dynamics. To address this, we experimentally excluded insectivorous vertebrate predators (e.g. birds, bats) and measured leaf traits associated with herbivory in 52 populations of 12 oak (Quercus) species in three island–mainland sites: The Channel Islands of California vs. mainland California, Balearic Islands vs. mainland Spain, and the island Bornholm vs. mainland Sweden (N = 204 trees). In each site, at the end of the growing season, we measured leaf damage by insect herbivores on control vs. predator-excluded branches and measured leaf traits, namely: phenolic compounds, specific leaf area, and nitrogen and phosphorous content. In addition, we obtained climatic and soil data for island and mainland populations using global databases. Specifically, we tested for island–mainland differences in herbivory, and whether differences in vertebrate predator effects or leaf traits between islands and mainland contributed to explaining the observed herbivory patterns. Supporting predictions, herbivory was lower on islands than on mainland, but only in the case of Mediterranean sites (California and Spain). We found no evidence for vertebrate predator effects on herbivory on either islands or mainland in any study site. In addition, while insularity affected leaf traits in some of the study sites (Sweden-Bornholm and California), these effects were seemingly unrelated to differences in herbivory. Synthesis. Our results suggest that vertebrate predation and the studied leaf traits did not contribute to island–mainland variation patterns in herbivory, calling for more nuanced and comprehensive investigations of predator and plant trait effects, including measurements of other plant traits and assessments of predation by different groups of natural enemies.This research was supported by a grant for early career postdoctoral researchers from the Spanish Association of Terrestrial Ecology (AEET) to Carla Vázquez-González, two grants from the Spanish Ministry of Science and Innovation (PID2022-141761OB-I00, EUR2023-143463) to Xoaquín Moreira, a grant from Galician Innovation Agency (Oportunius Program) to Xoaquín Moreira, a grant from the Regional Government of Galicia (IN607A 2021/03) to Xoaquín Moreira and Carla Vázquez-González, and two NSF grants (DEB-2032435, IOS-1951244) to Kailen A. Mooney. Carla Vázquez-González was supported by a postdoctoral fellowship from the Xunta de Galicia-GAIN/ Fulbright Spain (IN606B 2021/004). Ayco J.M. Tack was supported by the Swedish Research Council (2015-03993 and 2019-04493). Johan A. Stenberg was supported by the SLU Centre for Biological Control.peerReviewe