Genome Skimming of Thysanoptera (Arthropoda, Insecta) and Its Taxonomic and Systematic Applications

Abstract

High-throughput sequencing has transformed molecular systematics. This study presents a semi-automated pipeline for genome skimming in Thysanoptera, an insect order known for challenging species identification and cryptic relationships. By efficiently obtaining mitochondrial genomes and nuclear genes from multiple thrips specimens, the study evaluates the limitations of traditional barcoding and the data required for accurate species delimitation. The results highlight the importance of the sequencing data volume and this pipeline in reconstructing Thysanoptera phylogeny. This research also showcases the potential of advanced sequencing techniques for species delimitation and phylogenetics