Description of Corynebacterium hiratae sp. nov. isolated from a human tissue bone a novel member of Corynebacterium Genus

Abstract

Produção científica do Laboratório Interdisciplinar de Pesquisas Médicas.This work was supported by Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) # E-17/205.900/2022 and # E-26/202.088/2020.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) # 309948/2018-5.Secretaria de Ciencia y Técnica de la Universidad de Buenos Aires (UBACyT) # 20020170100109 BA.Background: Corynebacterium spp. are widely disseminated in the environment, and they are part of the skin and mucosal microbiota of animals and humans. Reports of human infections by Corynebacterium spp. have increased considerably in recent years and the appearance of multidrug resistant isolates around the world has drawn attention. Objectives: To describe a new species of Corynebacterium from human tissue bone is described after being misidentified using available methods. Methods: For taxonomic analyses, phylogenetic analysis of 16S rRNA and rpoB genes, in silico DNA-DNA hybridization, average nucleotide and amino acid identity, multilocus sequence analysis, and phylogenetic analysis based on the complete genome were used. Findings: Genomic taxonomic analyzes revealed values of in silico DNA-DNA hybridization, average nucleotide and amino acids identity below the values necessary for species characterization between the analyzed isolates and the closest phylogenetic relative Corynebacterium aurimucosum DSM 44532ᵀ. Main conclusions: Genomic taxonomic analyzes indicate that the isolates analyzed comprise a new species of the Corynebacterium genus, which we propose to name Corynebacterium hiratae sp. nov. with isolate 332ᵀ (=CBAS 826ᵀ=CCBH 35,014ᵀ) as the type strain