THE EFFECT OF CANNABINOIDS ON THE DEVELOPMENTAL MORPHOLOGY OF ZEBRAFISH: A SUMMARY**

Abstract

Results from the past few years on exposure of zebrafish embryos to varying concentrations of delta nine-tetrahydrocannabinol (Δ9-THC), Δ8-THC and cannabidiol demonstrated delayed hatch rate, higher death rate, lowered heart rates and shorter embryos with curved spines when compared to control embryos. We also observed alterations on morphology of jaw structures though have yet to see a consistent pattern. In this study we are processing embryos from last year stored in 100% methanol and are using Alcian Blue staining to examine jaw structure in an effort to identify a consistent pattern of abnormality. We will also analyze data across all three years to summarize overarching trends