Optimization study for enhanced production of hyaluronic acid from Streptococcus equisimilus MK156140

Abstract

Hyaluronic acid finds its complete application in areas such as therapeutics, cosmetics, and as a health supplement. In the present investigation, standardization for the production of hyaluronic acid by Streptococcus equisimilus MK156140 in complex media was performed. Some of the selected physicochemical parameters such as pH, temperature, speed, incubation time, sucrose, yeast extract, and beef extract were screened using Plackett-Burman foldover design. Further, the screened parameters interaction was investigated using central composite design (CCD) and closely compared with OVAT studies. At a pH of 7.38, with beef extract, 12.15%, and yeast extract 7.64%, the observed yield was 7.16 g/L, which was in close line with the predicted value of 7.21 g/L