ABSTRACT: Adulteration of honey is a major problem in the world, due to its high nutritional value and the
expensive cost of honey. Thus, the quality of honey produced in different regions must be assessed to protect the
rights of consumers. The study aims to investigate the physicochemical (hydroxymethylfurfural: HMF, moisture, ash,
electrical conductivity, pH, total acidity, diastase activity, and reduction sugar), and microbiological (clostridium
perfringens, molds, and osmotolerant yeasts) parameters of 43 honey samples. All the honey samples were collected
from Qazvin province, Iran. Our results demonstrate that pH and acidity values in all of the honey samples were in the
accepted limit and other physicochemical parameters include HMF (44.18%), reduction sugar (9.30%), moisture
(2.32%), sucrose (53.48%), diastase activity (58.13%), fructose/glucose ratio (25.58%), electrical conductivity
(9.30%) and ash (4.65%) were below the acceptable quality level. All the honey samples were in the acceptable range
in terms of microbial quality (yeast, fungi and, Clostridia). All the honey samples are within expected microbial levels
but in non-standard physicochemical conditions. Our results indicate that you can use fast, inexpensive and safe tests
for identifying the adulteration in a variety of honeys (commercial and non-commercial). These measurements should
be widely practiced by governmental organizations determine a fair and reasonable price for each product
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