Biosynthesis of Iron Oxide Nanoparticles Using Ethyl Acetate Extract of Chaetomium Cupreum and their Anticancer Activity

Abstract

Background: The biosynthesis of iron oxide nanoparticle (NP) formation was carried out using ethyl acetate extract of fungus Chaetomium cupreum as reducing agents. The C. cupreum contains azaphilones pigments which poses various biological activities. Objectives: The synthesis of iron oxide NP and their anticancer potential was investigated. Materials and Methods: The anticancer activities of biosynthesized iron oxide NP were evaluated using tetrazolium bromide assay, measurement of reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and inhibition of tumorsphere formation. Results: In the present study, the X-ray diffraction shows the presence of gamma phase iron oxide NP withe the type of Fe2O3. The anticancer potential of iron oxide NP was investigated against human breast cancer cell line. The anticancer activity of biosynthesized iron oxide NP against MCF-7 was 20.5, 30.5, 41.1, 55.3 67.5, and 75.25 at 50 μg/ml after 1, 5, 10, 15, 20, and 24 h of treatment, respectively. The results showed that Fe2O3NP induced ROS generation to 68.22% at the concentration of 25 μg/ml and 83.66% at 50 μg/ml as compared to 48.22 in control after 15 h of treatment. The results showed that Fe2O3NP treatment increased depolarization MMP to 8.52% at 25 μg/ml and 10.74% at 50 μg/ml as compared to 6.35% in untreated cells after 24 h. Thus, treatment with Fe2O3NPs showed significant inhibition of MCF-7 tumorsphere formation at higher concentration. Conclusion: The biosynthesized iron oxide NP using ethyl acetate extract of C. cupreum exhibit significant anticancer activity