Reverse QMRA As a Decision Support Tool: Setting Acceptable Concentration Limits for Pseudomonas Aeruginosa and Naegleria Fowleri

Abstract

Opportunistic premise plumbing pathogens such as Pseudomonas aeruginosa and Naegleria fowleri are a growing concern in building water systems because of their potential risks to human health. The aim of this study is to determine the critical concentrations of P. aeruginosa and N. fowleri in water that are associated with meaningful public health risks. To determine these concentrations, a reverse quantitative microbial risk assessment (QMRA) was conducted. Environmental concentrations of P. aeruginosa and N. fowleri corresponding to the risk target of 1 micro- disability-adjusted life year (DALYs) per person per year and 1∗10−4 annual risks of illness were calculated for several applicable exposure scenarios. To calculate the concentration of P. aeruginosa, cleaning contact lenses with potentially contaminated tap water in the absence of an appropriate cleaning solution was considered. For N. fowleri, two exposure scenarios, recreational exposure (swimming), and nasal cleansing (via the use of a Neti pot or similar device) were considered. Critical mean concentrations of P. aeruginosa were obtained (mean concentration 0.30 CFU/L to 0.33 CFU/L) for the two experimentally measured and drop volume exposure methods based on the DALY approach. The highest critical concentration for P. aeruginosa was found to be 33.41 CFU/L with a 95% confidence interval of [2.03,117.70] for the drop measurement exposure method using the 10-4 annual risk target. For N. fowleri, based on the DALY approach, critical concentrations were 1.08 N. fowleri/L for swimming and 0.02 N. fowleri/L for Neti pot use scenario. Considering the heat inactivation, the critical concentration limits for P. aeruginosa using the DALY approach and the 10-4 annual risk target approach were found 0.55 CFU/L and 54.57 CFU/L, respectively based on the measured scenario. For N. fowleri, the 10-4 annual risk target approach resulted in 20.95 N. fowleri/L and the DALY approach resulted in 0.02 N. fowleri/L for the Neti pot scenario. Sensitivity analysis was performed for all the exposure scenarios. For P. aeruginosa, N50 and alpha (α) contributed the most and contact rates the least to the variability and uncertainty of the estimates for all the scenarios. For N. fowleri, N50 and contact rates contributed the most and α the least to the variability and uncertainty to calculate the concentrations for all the scenarios. The QMRA framework implemented in this research can be used to incorporate more information regarding opportunistic pathogens to inform management decisions, and to prioritize the best intervention regarding estimated reduction in infections caused by opportunistic pathogens.M.S., Environmental Engineering -- Drexel University, 201