First report of a novel alphapartitivirus in the basidiomyceteRhizoctonia oryzae?sativae

Abstract

AbstractRhizoctonia oryzae-sativae is a soil-borne basidiomycete fungus that causes aggregate sheath spot disease on rice worldwide.Here, we report the complete genome sequence of a partitivirus designated as Rhizoctonia oryzae-sativae partitivirus 1(RosPV1) infecting this fungus. The genome of RosPV1 consists of two double-stranded RNA (dsRNA) segments. The largersegment, designated as dsRNA-1 (1,961 bp), contains a single open reading frame (ORF) that encodes a putative polypeptidewith a conserved RNA-dependent RNA polymerase (RdRp) domain. The smaller segment, dsRNA-2 (1,819 bp), also hasa single ORF, which is predicted to encode the capsid protein (CP). BLAST searches and phylogenetic analyses suggestedthat RosPV1 is a representative member of a new species within the genus Alphapartitivirus. This is the first report of analphapartitivirus infecting the fungus R. oryzae-sativae