Viral metagenomics reveals significant viruses in the genital tractof apparently healthy dairy cows

Abstract

AbstractThe virome in genital tract secretion samples collected from 80 dairy cattle in Shanghai, China, was characterized. Virusesdetected included members of the families Papillomaviridae, Polyomaviridae, Hepeviridae, Parvoviridae, Astroviridae,Picornaviridae, and Picobirnaviridae. A member of a new species within the genus Dyoxipapillomavirus and six circularRep-encoding single-stranded DNA (ssDNA) (CRESS-DNA) viral genomes were fully sequenced and phylogeneticallyanalyzed. The prevalence of bovine polyomaviruses 1 and 2 was measured by PCR to be 10% (8/80) and 6.25% (5/80),respectively. PCR screening also indicated that the novel papillomavirus ujs-21015 and bovine herpesvirus 6 were presentin three and two out of the 80 samples, respectively