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Calcium-phosphate, DEAE-dextran co-precipitation, and electroporation to transfer genes into neuronal and glial cell lines

By M. G. Castro, J. Rowe, E. Morrison, Peter Tomasec, C. A. Murray, A. F. Shering and P. R. Lowenstein

Abstract

The brain and the cells that compose it have always presented unique barriers to any kind of outside interventions, including drug delivery, surgical procedures and the genetic manipulation of cells. However, these barriers are now being bypassed by the new technology of gene delivery. Protocols for Gene Transfer in Neuroscience is the first practical guide to gene delivery, providing over 150 easy to follow, step-by-step protocols, together with troubleshooting hints and the theoretical background needed for neuroscientists to understand, adapt and expand the technology as neede

Topics: R1
Publisher: John Wiley & Sons
Year: 1995
OAI identifier: oai:http://orca.cf.ac.uk:31676
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