The formulation of a competitive strategy implies an extended understanding, in terms of the industrial structures, of the mains fields where the nations compete and those structures evolve. The environmental conditions of a region, and of its industries, determine both the generic strategies, and the alternative strategies, which are already implemented. One of the instruments for strategic analysis, which combines external variables and internal variables, is the TOWS Matrix. This instrument allows the analysis of the present strategies, and the relationship between the variables, and also the presentation of proposals for alternative strategies, in order to identify or to reinforce the competitive advantages of the unit of analysis. Considering as unit of analysis the region of Beira Interior (Portugal), this article aims to provide a TOWS Matrix application. Several strategic alternatives for the region are, also, presented, taking into consideration the opportunities and the forces, previously, detected, in order to assure the transition for the ideal strategic quadrant. Finally, the conclusions and the guidelines for future research are presented.Strategy, TOWS Matrix, Entrepreneurship, Innovation.