The Effects of the Synthetic Antioxidant, Tempol, on Serum Glucose and Lipid Profile of Diabetic and Non-Diabetic Rats

Abstract

Background Hyperlipidemia and low antioxidant levels is one the diabetes side effects. Some studies have indicated the possible effects of nutrients on the improvement of hyperlipidemia, by their antioxidants ingredients. Objectives The aim of the present study was to evaluate the effect of the synthetic antioxidant, tempol, on blood lipid profiles and glucose levels in healthy and diabetic rats. Materials and Methods Adult Wistar rats were randomly divided to four experimental groups including, healthy control, diabetic control, diabetic receiving tempol and healthy receiving tempol groups. Diabetes was induced by injection of streptozotocin (60 mg/kg, Intraperitoneally (IP)). The rats were then fed saline or tempol (30 mg/kg) by gavage for 60 days. Blood samples were collected by cardiac puncture. Next, glucose, high-density lipoprotein (HDL), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), cholesterol, triglyceride and HbA1c were measured by specific kits. Also, the coronary risk index was calculated. Results The blood glucose level increased following diabetes induction. The level of blood glucose in the diabetic receiving tempol group decreased compared to the control diabetic group. The comparison of LDL, VLDL, cholesterol, triglyceride, HbA1c and coronary risk index among experimental groups indicated the increase of these factors in the diabetic group. High-density lipoprotein in the diabetic groups was lower than the other groups. Conclusions It can be concluded that tempol can improve dyslipidemia and may decrease hyperglycemia in diabetes. It seems that antioxidants such as tempol can improve dyslipidemia and may decrease hyperglycemia in diabetes