Continuously monitoring cell cultures is essential for both controlling
critical parameters and improving understanding of key processes. An ideal
technique in this context is surface plasmon resonance (SPR) spectroscopy,
which essentially exploits changes in the angle of incident light that occur
when molecules bind to a surface. It provides the ability to monitor
real-time changes in small concentrations of various molecules, with no need
for additional labels or sample preparation. Here we present an SPR-based
immunoassay for monitoring concentrations of human serum albumin (HSA), and
compare its sensitivity when used in conjunction with a Biacore platform and
the cheaper, smaller liSPR system. In conjunction with either
system, the immunoassay can detect HSA (a hepatocyte viability marker) at
concentrations typically present in three-dimensional hepatocyte cultures
mimicking the liver used to evaluate effects of drug candidates before
exposure to humans or animals. Furthermore, in conjunction with the
liSPR system, it is sufficiently sensitive to measure the much
lower HSA levels present in skin–hepatocyte co-cultures
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