research article
oaioai:doaj.org/article:3b1a070de29e4e9b9bd0d89dd166b1c7

HPLC Quantification of Cytotoxic Compounds from Aspergillus niger

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Publication date
1 January 2017
Publisher
Wiley
Doi

    Abstract

    A high-performance liquid chromatography method was developed and validated for the quantification of the cytotoxic compounds produced by a marine strain of Aspergillus niger. The fungus was grown in malt peptone dextrose (MPD), potato dextrose yeast (PDY), and mannitol peptone yeast (MnPY) media during 7, 14, 21, and 28 days, and the natural products were identified by standard compounds. The validation parameters obtained were selectivity, linearity (coefficient of correlation > 0.99), precision (relative standard deviation below 5%), and accuracy (recovery > 96)

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