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The Maize PIN Gene Family of Auxin Transporters

By Cristian Forestan, Silvia Farinati and Serena Varotto


Auxin is a key regulator of plant development and its differential distribution in plant tissues, established by a polar cell to cell transport, can trigger a wide range of developmental processes. A few members of the two families of auxin efflux transport proteins, PIN-formed (PIN) and P-glycoprotein (ABCB/PGP), have so far been characterized in maize. Nine new Zea mays auxin efflux carriers PIN family members and two maize PIN-like genes have now been identified. Four members of PIN1 (named ZmPIN1a–d) cluster, one gene homologous to AtPIN2 (ZmPIN2), three orthologs of PIN5 (ZmPIN5a–c), one gene paired with AtPIN8 (ZmPIN8), and three monocot-specific PINs (ZmPIN9, ZmPIN10a, and ZmPIN10b) were cloned and the phylogenetic relationships between early-land plants, monocots, and eudicots PIN proteins investigated, including the new maize PIN proteins. Tissue-specific expression patterns of the 12 maize PIN genes, 2 PIN-like genes and ZmABCB1, an ABCB auxin efflux carrier, were analyzed together with protein localization and auxin accumulation patterns in normal conditions and in response to drug applications. ZmPIN gene transcripts have overlapping expression domains in the root apex, during male and female inflorescence differentiation and kernel development. However, some PIN family members have specific tissue localization: ZmPIN1d transcript marks the L1 layer of the shoot apical meristem and inflorescence meristem during the flowering transition and the monocot-specific ZmPIN9 is expressed in the root endodermis and pericycle. The phylogenetic and gene structure analyses together with the expression pattern of the ZmPIN gene family indicate that subfunctionalization of some maize PINs can be associated to the differentiation and development of monocot-specific organs and tissues and might have occurred after the divergence between dicots and monocots

Topics: Plant Science
Publisher: Frontiers Research Foundation
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Provided by: PubMed Central
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    1. (2000). A floret byanyothername:controlof meristem identity in maize.
    2. (2011). accepted: 17
    3. (1999). Anauxin-dependentdistalorganizer of pattern and polarity in the Arabidopsis root.
    4. and Kuhlemeier,C.(2003).Regulationof phyllotaxisbypolarauxintransport.
    5. and Luo,J.C.(2007).GSDS:agenestructure display server.
    6. (2006). Application of phylogenetic networks in evolutionary studies.
    7. (2010). Approaching cellular and molecular resolution of auxin biosynthesis and metabolism.
    8. (2008). Auxin acts as a local morphogenetic trigger to specify lateral root founder cells.
    9. (2006). Auxin immunolocalization implicatesvesicularneurotransmitter-like mode of polar auxin transport in root apices.
    10. (2000). Auxin regulates the initiation and radial position of plant lateral organs.
    11. (2006). Auxin transport and gravitational research: perspectives.
    12. (2009). Auxin transport routes in plant development.
    13. (2010). Auxin transporters – why so many? Cold Spring Harb.
    14. (2010). Brachytic2/ZmABCB1 functions in IAA export from intercalary meristems.
    15. (2010). Control of leaf and vein development by auxin. Cold Spring Harb.
    16. (2006). Control of leaf vascular patterning by polar auxin transport.GenesDev.20,1015–1027.
    17. (1997). DNA sequence evidence for the segmental allotetraploid origin of maize.
    18. (2003). Efflux-dependent auxin gradients establishtheapical-basalaxisofArabidopsis.
    19. (2009). Expression of PIN genes in rice (Oryza sativa L.): tissue specificity and regulation by hormones.
    20. (2004). From weeds to crops: genetic analysis of root development in cereals.
    21. (2009). Functional expression and characterizationofArabidopsisABCB,AUX 1 and PIN auxin transporters in Schizosaccharomyces pombe.
    22. (2011). Genome-wide atlas of transcription during maize development.
    23. (1988). Identification of the genomic locations of duplicate nucleotide sequences in maize by analysis of restriction fragment length polymorphisms.
    24. (2002). Indole acetic acid distribution coincides with vascular differentiation pattern during Arabidopsis leaf ontogeny.
    25. (2005). involved in auxin-dependent adventitious root emergence and tillering in rice.
    26. (2007). Isolation and characterization of a new mutant allele of brachytic 2 maize gene.
    27. (2005). Isolation,characterization,andpericyclespecific transcriptome analyses of the novel maize lateral and seminal root initiation mutant rum1. Plant Physiol.
    28. (2003). Local,efflux-dependentauxingradients as a common module for plant organ formation.
    29. (2003). localization of maize Rpd3-type histone deacetylases during plant development.
    30. (2003). Loss of an MDR transporter in compact stalksofmaizebr2andsorghumdw3 mutants.
    31. (2002). MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform.
    32. (2005). Maintenance of embryonic auxin distribution for apicalbasal patterning by PIN-FORMEDdependent auxin transport in Arabidopsis.
    33. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionarydistance,andmaximumparsimony methods.
    34. (2007). Molecular and cellular aspects of auxin-transportmediateddevelopment.TrendsPlant Sci.
    35. (2009). Multiple alignment of DNA sequences with MAFFT.
    36. (2002). Multiple sequence alignment using ClustalW and ClustalX.
    37. (2007). New insight into the biochemical mechanisms regulating auxin transport in plants.
    38. (2010). Non-invasive quantification of endogenous root auxin transport using an integrated flux microsensor technique.
    39. (2005). Patterns of auxin transport and gene expression during primordium development revealed by live imaging of the Arabidopsis inflorescence meristem.
    40. (2006). Phototropin and light-signaling in phototropism.
    41. (1989). PHYLIP – phylogeny inference package.
    42. (2010). PIN1 auxin efflux carriers localization studies in Zea mays.
    43. (2006). PINproteinsperformarate-limiting function in cellular auxin efflux.
    44. (2008). Plant ABC proteins – a unified nomenclature and updated inventory.
    45. Polar transport of the plant hormone auxin – the role of PINFORMED (PIN) proteins.
    46. (2008). Post-transcriptional regulationof auxintransportproteins:cellulartrafficking,proteinphosphorylation, protein maturation, ubiquitination, and membrane composition.
    47. (2001). Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
    48. (2011). Prototype cell-to-cell auxin transport mechanismbyintracellularauxincompartmentalization.
    49. (2010). Role of the GNOM gene in Arabidopsis apicalbasal patterning–from mutant phenotype to cellular mechanism of protein action.
    50. S.(2004).Relocalizationof thePIN1 auxin efflux facilitator plays a role in phototropicresponses.PlantPhysiol.
    51. (2011). Seven things wethinkweknowaboutauxintransport.
    52. (2006). Spatiotemporal asymmetric auxin distribution: a means to coordinate plant development. C e l l .M o l .L i f eS c i .63,
    53. (2009). Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter.
    54. (2011). SyMAP v3.4: a turnkey synteny system with application to plant genomes.
    55. (2009). The B73 maize genome: complexity, diversity, and dynamics.
    56. (2005). The fall and rise of apical dominance.
    57. (2006). The genome of black cottonwood,
    58. (2004). The maize duplicate genes narrow sheath1 and narrow sheath2 encode a conserved homeobox gene function in a lateral domain of shoot apical meristems.
    59. (2005). The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots.
    60. (2005). The PIN auxin efflux facilitators: evolutionary and functional perspectives.
    61. (2009). The PINFORMED (PIN) protein family of auxin transporters.
    62. (2008). The Relationship between auxin transport and maize branching.
    63. (2010). The role of PIN auxin efflux carriers in polar auxin transport and accumulation and their effect on shaping maize development.
    64. (2006). The shoot stem cell niche in angiosperms: expression patterns of WUS orthologues in rice and maize imply major modifications in the course of mono- and dicot evolution.
    65. (2006). TheABCof auxintransport:therole of p-glycoproteins in plant development.
    66. (2010). ZmPIN1-mediated auxin transport is related to cellular differentiation during maize embryogenesis and endosperm development.
    67. (2006). ZmPIN1a and ZmPIN1b encode two novel putative candidates for polar auxin transport and plant architecture determination of maize.

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