Skip to main content
Article thumbnail
Location of Repository

Suppression of zinc finger protein 467 alleviates osteoporosis through promoting differentiation of adipose derived stem cells to osteoblasts

By Li You, Ling Pan, Lin Chen, Jin-Yu Chen, Xiaoping Zhang, Zhongwei Lv and Da Fu

Abstract

Osteoblast and adipocyte are derived from common mesenchymal progenitor cells. The bone loss of osteoporosis is associated with altered progenitor differentiation from an osteoblastic to an adipocytic lineage. In this study, a comparative analysis of gene expression profiling using cDNA microarray and realtime-PCR indicated that Zinc finger protein 467 (Zfp467) involved in adipocyte and osteoblast differentiation of cultured adipose derived stem cells (ADSCs). Our results showed that RNA interference for Zfp467 in ADSCs inhibited adipocyte formation and stimulated osteoblast commitment. The mRNA levels of osteogenic and adipogenic markers in ADSCs were regulated by si-Zfp467. Zfp467 RNAi in ADSCs could restore bone function and structure in an ovariectomized (OVX)-induced osteoporotic mouse model. Thus Zfp467 play an important role in ADSCs differentiation to adipocyte and osteoblast. This has relevance to therapeutic interventions in osteoporosis, including si-Zfp467-based therapies currently available, and may be of relevance for the use of adipose-derived stem cells for tissue engineering

Topics: Research
Publisher: BioMed Central
OAI identifier: oai:pubmedcentral.nih.gov:3293781
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    Citations

    1. (2008). Abdallah BM: Human bone-marrow-derived mesenchymal stem cells: biological characteristics and potential role in therapy of degenerative diseases. Cell Tissue Res
    2. (1999). Abnormal osteogenesis in osteoporotic patients is reflected by altered mesenchymal stem cells dynamics.
    3. (2011). Adipose-derived stromal/stem cells (ASC) in regenerative medicine: pharmaceutical applications. Curr Pharm Des
    4. (2008). AM: Involvement of adipogenic potential of human bone marrow mesenchymal stem cells (MSCs) in osteoporosis. Curr Stem Cell Res Ther
    5. (2008). Batra SK: Recent progress on tissue-resident adult stem cell biology and their therapeutic implications. Stem Cell Rev
    6. (2010). Bouxsein ML: Role of trabecular microarchitecture and its heterogeneity parameters in the mechanical behavior of ex vivo human L3 vertebrae. J Bone Miner Res
    7. (2003). Canalis E: Osteonectin-null mutation compromises osteoblast formation, maturation, and survival. Endocrinology
    8. (2002). Dietary essential amino acid supplements increase bone strength by influencing bone mass and bone microarchitecture in ovariectomized adult rats fed an isocaloric low-protein diet. J Bone Miner Res
    9. (1999). DR: Multilineage potential of adult human mesenchymal stem cells. Science
    10. (2001). Hedrick MH: Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng
    11. (2004). Inhibition of growth and differentiation of osteoprogenitors in mouse bone marrow stromal cell cultures by increased donor age and glucocorticoid treatment.
    12. (2006). Laharrague P: Human subcutaneous adipose cells support complete differentiation but not self-renewal of hematopoietic progenitors.
    13. (1996). Manolagas SC: Linkage of decreased bone mass with impaired osteoblastogenesis in a murine model of accelerated senescence.
    14. (2009). Papavassiliou AG: Mechanotransduction in osteoblast regulation and bone disease. Trends Mol Med
    15. (2005). Pathogenesis of osteoporosis: concepts, conflicts, and prospects.
    16. (2008). Pino AM: Involvement of adipogenic potential of human bone marrow mesenchymal stem cells (MSCs) in osteoporosis. Curr Stem Cell Res Ther
    17. (2009). Restoration of bone mass and strength in glucocorticoid-treated mice by systemic transplantation of CXCR4 and cbfa-1 co-expressing mesenchymal stem cells. J Bone Miner Res
    18. (2010). Richards PJ: Influence of defective bone marrow osteogenesis on fracture repair in an experimental model of senile osteoporosis.
    19. (2008). SK: Functions of normal and malignant prostatic stem/progenitor cells in tissue regeneration and cancer progression and novel targeting therapies. Endocr Rev
    20. (2007). Skeletal remodeling in health and disease. Nat Med
    21. (2011). Systemic transplantation of human adipose-derived stem cells stimulates bone repair by promoting osteoblast and osteoclast function.
    22. (1998). T: Reduced expression of interleukin-11 in bone marrow stromal cells of senescence accelerated mice (SAMP6): Relationship to osteopenia with enhanced adipogenesis. J Bone Miner Res
    23. (2011). TJ: Zinc finger protein 467 is a novel regulator of osteoblast and adipocyte commitment.
    24. (2008). Transient receptor potential vanilloid 4 deficiency suppresses unloading-induced bone loss.
    25. (2003). WL: Mesenchymal progenitor self-renewal deficiency leads to agedependent osteoporosis in Sca-1/Ly-6A null mice. Proc Natl Acad Sci USA
    26. (2006). Y: v-ATPase V0 subunit d2-deficient mice exhibit impaired osteoclast fusion and increased bone formation. Nat Med

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.