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Role of Mesenchymal Stem Cells on Cornea Wound Healing Induced by Acute Alkali Burn

By Lin Yao, Zhan-rong Li, Wen-ru Su, Yong-ping Li, Miao-li Lin, Wen-xin Zhang, Yi Liu, Qian Wan and Dan Liang

Abstract

The aim of this study was to investigate the effects of subconjunctivally administered mesenchymal stem cells (MSCs) on corneal wound healing in the acute stage of an alkali burn. A corneal alkali burn model was generated by placing a piece of 3-mm diameter filter paper soaked in NaOH on the right eye of 48 Sprague-Dawley female rats. 24 rats were administered a subconjunctival injection of a suspension of 2×106 MSCs in 0.1 ml phosphate-buffered saline (PBS) on day 0 and day 3 after the corneal alkali burn. The other 24 rats were administered a subconjunctival injection of an equal amount of PBS as a control. Deficiencies of the corneal epithelium and the area of corneal neovascularization (CNV) were evaluated on days 3 and 7 after the corneal alkali burn. Infiltrated CD68+ cells were detected by immunofluorescence staining. The mRNA expression levels of macrophage inflammatory protein-1 alpha (MIP-1α), tumor necrosis factor-alpha (TNF-α), monocyte chemotactic protein-1 (MCP-1) and vascular endothelial growth factor (VEGF) were analyzed using real-time polymerase chain reaction (real-time PCR). In addition, VEGF protein levels were analyzed using an enzyme-linked immunosorbent assay (ELISA). MSCs significantly enhanced the recovery of the corneal epithelium and decreased the CNV area compared with the control group. On day 7, the quantity of infiltrated CD68+ cells was significantly lower in the MSC group and the mRNA levels of MIP-1α, TNF-α, and VEGF and the protein levels of VEGF were also down-regulated. However, the expression of MCP-1 was not different between the two groups. Our results suggest that subconjunctival injection of MSCs significantly accelerates corneal wound healing, attenuates inflammation and reduces CNV in alkaline-burned corneas; these effects were found to be related to a reduction of infiltrated CD68+ cells and the down-regulation of MIP-1α, TNF-α and VEGF

Topics: Research Article
Publisher: Public Library of Science
OAI identifier: oai:pubmedcentral.nih.gov:3281878
Provided by: PubMed Central
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    1. (2006). Alkali-induced corneal stromal melting prevention by a novel platelet-activating factor receptor antagonist.
    2. (1999). Angiogenesis and ophthalmic disease.
    3. (2003). Bone marrow mesenchymal stem cells inhibit the response of naive and memory antigenspecific T cells to their cognate peptide.
    4. (1997). Chemical injuries of the eye: current concepts in pathophysiology and therapy.
    5. (2004). Corneal wound healing.
    6. (2005). Deep anterior lamellar keratoplasty combined with autologous limbal stem cell transplantation in unilateral severe chemical injury.
    7. (2011). Effects of amniotic membrane suspension in the rat alkali burn model.
    8. (2002). Emergency treatment of chemical and thermal eye burns.
    9. (2010). Human gingiva-derived mesenchymal stem cells elicit polarization of m2 macrophages and enhance cutaneous wound healing.
    10. (2011). Human gingiva-derived mesenchymal stromal cells attenuate contact hypersensitivity via prostaglandin E(2) -dependent mechanisms.
    11. (2007). Human heart, spleen, and perirenal fat-derived mesenchymal stem cells have immunomodulatory capacities.
    12. (2005). Human mesenchymal stem cells modulate allogeneic immune cell responses.
    13. (2005). Human mesenchymal stem cells require monocyte-mediated activation to suppress alloreactive T cells.
    14. (2008). Immunological properties of umbilical cord blood-derived mesenchymal stromal cells.
    15. (2010). Immunomodulatory properties of mesenchymal stromal cells and their therapeutic consequences for immune-mediated disorders.
    16. (2007). Immunomodulatory properties of mesenchymal stromal cells.
    17. (2000). Management of alkali burns: an 11-year retrospective review.
    18. (2010). Management of ocular thermal and chemical injuries, including amniotic membrane therapy.
    19. (2009). Management of severe ocular burns with symblepharon.
    20. (2006). Mesenchymal stem cell transplantation in a rabbit corneal alkali burn model: engraftment and involvement in wound healing.
    21. (2009). Mesenchymal stem cells derived from human gingiva are capable of immunomodulatory functions and ameliorate inflammation-related tissue destruction in experimental colitis.
    22. (2011). Mesenchymal stem cells for acute lung injury: role of paracrine soluble factors.
    23. (2008). Mesenchymal stem cells in health and disease.
    24. (1998). MIP-1alpha as a critical macrophage chemoattractant in murine wound repair.
    25. (2010). Mouse bone marrow-derived mesenchymal stromal cells turn activated macrophages into a regulatory-like profile.
    26. (2008). Multipotential human adipose-derived stromal stem cells exhibit a perivascular phenotype in vitro and in vivo.
    27. (2007). No contribution of multipotent mesenchymal stromal cells to liver regeneration in a rat model of prolonged hepatic injury.
    28. (2005). Reconstruction of chemically burned rat corneal surface by bone marrow-derived human mesenchymal stem cells.
    29. (2010). Reconstruction of the corneal epithelium with induced marrow mesenchymal stem cells in rats.
    30. (2008). The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury.
    31. (2001). The corneal wound healing response: cytokine-mediated interaction of the epithelium, stroma, and inflammatory cells.
    32. (2011). The effect of doxycycline temperature-sensitive hydrogel on inhibiting the corneal neovascularization induced by BFGF in rats.
    33. (2003). Treatment of the sequelae of ocular burns using limbal transplantation.

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