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Genomic Restructuring in the Tasmanian Devil Facial Tumour: Chromosome Painting and Gene Mapping Provide Clues to Evolution of a Transmissible Tumour

By Janine E. Deakin, Hannah S. Bender, Anne-Maree Pearse, Willem Rens, Patricia C. M. O'Brien, Malcolm A. Ferguson-Smith, Yuanyuan Cheng, Katrina Morris, Robyn Taylor, Andrew Stuart, Katherine Belov, Chris T. Amemiya, Elizabeth P. Murchison, Anthony T. Papenfuss and Jennifer A. Marshall Graves


Devil facial tumour disease (DFTD) is a fatal, transmissible malignancy that threatens the world's largest marsupial carnivore, the Tasmanian devil, with extinction. First recognised in 1996, DFTD has had a catastrophic effect on wild devil numbers, and intense research efforts to understand and contain the disease have since demonstrated that the tumour is a clonal cell line transmitted by allograft. We used chromosome painting and gene mapping to deconstruct the DFTD karyotype and determine the chromosome and gene rearrangements involved in carcinogenesis. Chromosome painting on three different DFTD tumour strains determined the origins of marker chromosomes and provided a general overview of the rearrangement in DFTD karyotypes. Mapping of 105 BAC clones by fluorescence in situ hybridisation provided a finer level of resolution of genome rearrangements in DFTD strains. Our findings demonstrate that only limited regions of the genome, mainly chromosomes 1 and X, are rearranged in DFTD. Regions rearranged in DFTD are also highly rearranged between different marsupials. Differences between strains are limited, reflecting the unusually stable nature of DFTD. Finally, our detailed maps of both the devil and tumour karyotypes provide a physical framework for future genomic investigations into DFTD

Topics: Research Article
Publisher: Public Library of Science
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Provided by: PubMed Central

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  1. (2006). Allograft theory: Transmission of devil facial-tumour disease.
  2. (1998). An improved approach for construction of bacterial artificial chromosome libraries.
  3. (2005). Characterizing the chromosomes of the Australian model marsupial Macropus eugenii (tammar wallaby).
  4. (2008). Comparative lesion sequencing provides insights into tumor evolution.
  5. (2009). Conservation of a chromosome arm in two distantly related marsupial species.
  6. (2006). Construction of a highly enriched marsupial Y chromosome-specific BAC sub-library using isolated Y chromosomes.
  7. (1996). Construction of P1 artificial chromosome (PAC) libraries from lower vertebrates.
  8. (1992). Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer.
  9. (2007). Distribution and impacts of Tasmanian devil facial tumor disease.
  10. (1997). DNA methylation and genetic instability in colorectal cancer cells.
  11. (2005). Dynamics of mammalian chromosome evolution inferred from multispecies comparative maps.
  12. (2006). Emerging disease and population decline of an island endemic, the Tasmanian devil Sarcophilus harrisii.
  13. (2010). Evaluation of selective culling of infected individuals to control Tasmanian Devil Facial Tumor Disease.
  14. (2008). From cytogenetics to next-generation sequencing technologies: advances in the detection of genome rearrangements in tumors.
  15. (1985). G-banding evidence for a conserved complement in the Marsupialia.
  16. (2009). Gene synteny comparisons between different vertebrates provide new insights into breakage and fusion events during mammalian karyotype evolution.
  17. (1997). Genetic analysis by chromosome sorting and painting: phylogenetic and diagnostic applications.
  18. (2007). Genome of the marsupial Monodelphis domestica reveals innovation in non-coding sequences.
  19. (2011). Genome sequence of an Australian kangaroo, Macropus eugenii, provides insight into the evolution of mammalian reproduction and development.
  20. (2009). Heterogeneity in cancer: cancer stem cells versus clonal evolution.
  21. (2008). Identification of somatically acquired rearrangements in cancer using genomewide massively parallel paired-end sequencing.
  22. (1999). Karyotype relationships between four distantly related marsupials revealed by reciprocal chromosome painting.
  23. (2008). Life-history change in disease-ravaged Tasmanian devil populations.
  24. (1974). Mammalia I: Monotremata and Marsupialia. In:
  25. (2007). Managing an emerging disease in a threatened species: Tasmanian devil facial tumour disease.
  26. (2011). Massive genomic rearrangement acquired in a single catastrophic event during cancer development.
  27. (1998). Mice heterozygous for a mutation at the Nf2 tumor suppressor locus develop a range of highly metastatic tumors.
  28. (2009). Neurofibromatosis type 2.
  29. (2008). Physical map of two tammar wallaby chromosomes: a strategy for mapping in non-model mammals.
  30. (1967). Quantitative comparisons between karyotypes of Australian marsupials from 3 different superfamilies.
  31. (2008). Research priorities in the Tasmanian devil facial tumour debate.
  32. (2003). Reversal and convergence in marsupial chromosome evolution.
  33. (2000). Review of canine transmissible venereal sarcoma.
  34. (2006). Sarcophilus harrisii (Tasmanian Devil). In:
  35. (1999). Screening large-insert libraries by hybridization.
  36. (2008). Tasmanian devil facial tumour disease: lessons for conservation biology.
  37. (1982). The chromosomes of dasyurids (Marsupialia).
  38. (1976). The clonal evolution of tumor cell populations.
  39. (2007). The impact of disease on the survival and population growth rate of the Tasmanian devil.
  40. (2006). The pathology of devil facial tumor disease (DFTD) in Tasmanian Devils (Sarcophilus harrisii).
  41. (2004). The shortest telomeres drive karyotype evolution in transformed cells.
  42. (2010). The Tasmanian Devil Transcriptome Reveals Schwann Cell Origins of a Clonally Transmissible Cancer.
  43. (2007). Towards a case definition for devil facial tumour disease: What is it?
  44. (2007). Transmission of a fatal clonal tumor by biting occurs due to depleted MHC diversity in a threatened carnivorous marsupial.

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