Article thumbnail

microRNA-34a regulates neurite outgrowth, spinal morphology, and function

By Massimiliano Agostini, Paola Tucci, Joern R. Steinert, Ruby Shalom-Feuerstein, Matthieu Rouleau, Daniel Aberdam, Ian D. Forsythe, Kenneth W. Young, Andrea Ventura, Carla P. Concepcion, Yoon-Chi Han, Eleonora Candi, Richard A. Knight, Tak W. Mak and Gerry Melino

Abstract

The p53 family member TAp73 is a transcription factor that plays a key role in many biological processes, including neuronal development. In particular, we have shown that p73 drives the expression of miR-34a, but not miR-34b and c, in mouse cortical neurons. miR-34a in turn modulates the expression of synaptic targets including synaptotagmin-1 and syntaxin-1A. Here we show that this axis is retained in mouse ES cells committed to differentiate toward a neurological phenotype. Moreover, overexpression of miR-34a alters hippocampal spinal morphology, and results in electrophysiological changes consistent with a reduction in spinal function. Therefore, the TAp73/miR-34a axis has functional relevance in primary neurons. These data reinforce a role for miR-34a in neuronal development

Topics: Biological Sciences
Publisher: National Academy of Sciences
OAI identifier: oai:pubmedcentral.nih.gov:3248521
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.