Article thumbnail

Anti Microbial Resistance Profile of E. coli isolates From Tropical Free Range Chickens

By Charles Okoli I, Chah K F, Ozoh P TE and Udedibie A BI

Abstract

Normal intestinal flora of humans and animals constitute enormous reservoir of resistance genes for potentially pathogenic bacteria and may serve as major indictors of selection pressure exerted by anti-microbial use in a given population. A study was conducted in September 2003 at 3 purposively selected peri-urban sites spread across 3 senatorial zones of Imo state, Nigeria to determines the anti-microbial resistance profile of commensal E. coli isolated from free range chickens. The isolates were screened for anti-microbial resistance profile against 10 antibiotics using the disc diffusion method. E. coli strains from local fowls, recorded 100 and 78.9% resistances against ampicillin and cotrimoxazole respectively, while resistance rates against ciprofloxacin, gentamycin and norfloxacin were 0.0, 5.3 and 5.3% respectively. Isolates from free-range cockerels recorded 100% resistance against norfloxacin, cotrimoxazole and ampicillin and 83.3% against nitrofurantoin. Similarly, isolates from old layers, recorded 100% resistance against nitrofurantoin, cotrimoxazole, tetracycline, chloramphenicol and ampicillin and 80% against nalidixic acid. Similar resistance trends were observed in E. coli isolates from free-range turkeys and broiler roosters against the inexpensive broad-spectrum first line antibiotics (ampicillin, nalidixic acid, cotrimoxazole, nitrofurantoin and chloramphenicol), although values varied slightly across poultry types. Resistances against gentamycin were consistently low in isolates from the different types of poultry. Twenty-nine resistance patterns were observed in the E. coli isolates with predominant patterns being distributed widely across poultry types indicating a striking diversity of resistance patterns in the areas

Topics: Online Journal of Health and Allied Sciences
Publisher: Dr. B.S. Kakkilaya
Year: 2005
OAI identifier: oai:cogprints.org:4699

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles

Citations

  1. (2004). Aerial gases and temperature levels in selected poultry houses in Imo State - doi
  2. Agricultural use of antibiotics and the evolution and transfer of antibiotic resistant bacteria.
  3. Anti-microbial resistance of none clinical E. coli strains from chicken in Nsukka, Southeastern Nigeria. doi
  4. (2002). Anti-microbial use and anti-microbial resistance: A population perspective. Emerging Infectious Diseases
  5. (2000). Antibiotic resistance in avian colisepticeamic E. Coli strains in Southeast,
  6. Antibiotic susceptibility testing by a standardized single disk method.
  7. (1997). Bacteria in biology, biotechnology and medicine, 4th Ed.
  8. (1980). Drug resistance in salmonella isolated at Chandigarh (India) during 1972-1978. Antonie Van Leeuwenhoek
  9. (2000). Effect of antibiotic therapy on the density of vancomycin-resistant enteroccoci in the stool of colonized patients.
  10. Epidemiology of resistance to antibiotics. Links between animals and humans.
  11. (1972). Identification of Enterobacteriaceae, 3rd edition,
  12. (1998). Medical consequences of antibiotic use in agriculture. doi
  13. (2000). Microbiological tests. In: District laboratory practice in tropical countries. Part 2. Cambridge:
  14. (1996). Multi-drug resistant non-typhoid salmonellae in Kenya.
  15. (1999). NCCLS Performance standard of anti-microbial disk and dilution susceptibility tests for bacteria isolated from animals. Approved standards,
  16. Plasmid-mediated quinolone resistance in clinical isolates of E. coli from Shanghai, China. Antimirobial Agents Chemotherapy, doi
  17. (1992). Problems and Dilemmas of anti-microbial resistance.
  18. (1992). Reservoirs for Campylobacter jejuni and approaches for intervention in poultry. In: Nachamkin
  19. (1998). Role of veterinary therapeutics in bacterial resistance development: animal and public health perspectives.
  20. (1987). Salmonella serotypes and incidence of multipleresistant salmonellae isolated from diarrhoeal patients in Hong Kong from 1973-1982. Epidemiological of Infection
  21. (2003). Studies on the anti-microbial resistance of E. coli isolates from feeds and poultry production units. doi
  22. (1991). Study designs for dependent happenings.
  23. (1990). The carriage of Escherichia coli resistant to anti-microbial agents by healthy children in Boston,
  24. (1999). The use of antibiotics as growth promoters in animal husbandry and antibiotic resistance in bacterial pathogens of humans.
  25. (1998). Validity of Scal defined genotypes of Campylobacter jejuni examined by Scal I, Kp nl and Bam 1-11 polymorphisms: evidence of identical clones infecting humans, poultry and cattle. doi