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Estradiol Modulates Membrane-Linked ATPases, Antioxidant Enzymes, Membrane Fluidity, Lipid Peroxidation, and Lipofuscin in Aged Rat Liver

By Pardeep Kumar, R. K. Kale and Najma Zaheer Baquer


Free radical production and oxidative stress are known to increase in liver during aging, and may contribute to the oxidative damage. These changes increase during menopausal condition in females when the level of estradiol is decreased. The objective of this study was to observe the changes in activities of membrane linked ATPases (Na+K+ ATPase, Ca2+ ATPase), antioxidant enzymes (superoxide dismutase, glutathione-S-transferase), lipid peroxidation levels, lipofuscin content and membrane fluidity occurring in livers of female rats of 3, 12 and 24 months age groups, and to see whether these changes are restored to 3 months control levels rats after exogenous administration of 17-β-estradiol (E2). The aged rats (12 and 24 months) were given subcutaneous injection of E2 (0.1 μg/g body weight) daily for one month. The results obtained in the present work revealed that normal aging was associated with significant decrease in the activities of membrane linked ATPases, antioxidant enzymes, membrane fluidity and an increase in lipid peroxidation and lipofuscin content in livers of aging female rats. The present study showed that E2 treatment reversed the changes to normal levels. E2 treatment may be beneficial in preventing some of the age related changes in the liver by increasing antioxidant defenses

Topics: Research Article
Publisher: SAGE-Hindawi Access to Research
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Provided by: PubMed Central

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  1. (2006). A .K .M a n t h a ,K .M o o r t h y ,S .M .C o w s i k ,a n dN .Z .B a q u e r , “Membrane associated functions of neurokinin B (NKB) on Aβ (25–35) induced toxicity in aging rat brain synaptosomes,”
  2. (1973). A .T a p p e l ,B .F l e t c h e r ,a n dD .D e a m e r ,“ E ffect of antioxidants and nutrients on lipid peroxidation fluorescent products and aging parameters in the mouse,”
  3. (2008). A comparative study of artificial ceroid/lipofuscin from different tissue materials of rats,”
  4. (2009). A metabolic and functional overview of brain aging linked to neurological disorders,”
  5. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding,”
  6. (2010). Action of estrogens in the aging brain: dementia and cognitive aging,”
  7. (2005). Administration of estradiol and progesterone modulate the activities of antioxidant enzyme and aminotransferases in naturally menopausal rats,”
  8. (2009). Age-associated decrease of highdensity lipoprotein-mediated reverse cholesterol transport activity,”
  9. (2002). Alterations in antioxidant enzymes and oxidative damage in experimental diabetic rat tissues: effect of vanadate and fenugreek (Trigonella foenum graecum),”
  10. (2005). Amelioration of altered antioxidant status and membrane linked functions by vanadium and Trigonella in alloxan diabetic rat brains,”
  11. (2004). Antioxidant and antiapoptotic activities of idoxifene and estradiol in hepatic fibrosis
  12. (2008). as, “Modulation of longevity-associated genes by estrogens or phytoestrogens,”
  13. (2001). ATPase glutathione-S-transferase and multiple unit activity and reduces lipid peroxidation and lipofuscin concentration in aged rat brain regions,”
  14. (2011). Baquer,“Physiologicalandbiochemicaleffectsof17βestradiol in aging female rat brain,”
  15. (1994). C h e n ,N .H u ,a n dD .L .S n y d e r ,“ E ffects of age and dietary restriction on liver glutathione transferase activities
  16. (1991). Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes,”
  17. (1985). Chlordecone inhibition of calmodulin activated calcium ATPase in rat brain synaptosomes,”
  18. (1992). Differential effects of hemorrhage on kupffer cells: decreased antigen presentation despite increased inflammatory cytokine (IL-1,
  19. (1988). Drugs and the ageing liver,”
  20. (2008). Effect of dehydroepiandrosterone (DHEA) on monoamine oxidase activity, lipid peroxidation and lipofuscin accumulation in aging rat brain regions,”
  21. (2008). Effect of dehydroepiandrosterone (DHEA) onmonoamine oxidase activity, lipid peroxidation and lipofuscin accumulation in aging rat brain regions,”
  22. (2004). Effect of estradiol and progesterone treatment on carbohydrate metabolizing enzymes in tissues of aging female rats,”
  23. (2005). Effect of hormonereplacementtherapyinnormalizingagerelatedneuronal markers in different age groups of naturally menopausal rats,”
  24. (2009). Effect of Terminalia chebula aqueous extract on oxidative stress and antioxidant status in the liver and kidney of young and aged rats,”
  25. (2004). Effects of dietary restriction on age-related changes in the phospholipid fatty acid composition of various rat tissues,”
  26. (1999). Effects of partial hepatectomy on the plasma membrane status and the invertor mechanism of the hepatocyte Na,K-ATPase activity regulation in rats of various age,”
  27. (2005). Estrogen protects the liver and intestines against sepsisinduced injury in rats,”
  28. (2007). Feki, “Protective effect of 17β-estradiol on oxidative stress and liver dysfunction in aged male rats,”
  29. (2007). Fostering antioxidant defences: up-regulation of antioxidant genes or antioxidant supplementation?”
  30. (1992). Free radical theory of aging,”
  31. (2003). Glutathione S-transferase isoenzymatic response to aging in rat cerebral cortex and cerebellum,”
  32. (1999). GST function in drug and stress response,”
  33. Hormonal regulation of pro-inflammatory and lipid peroxidation processes in liver of old ovariectomizedfemale rats,”
  34. (2009). Inhibitory effect of estrogens, phytoestrogens, and caloric restriction on oxidative stress and hepato-toxicity in aged rats,”BiomedicalandEnvironmentalSciences,vol.22,no.5,pp. 381–387,
  35. (1974). Involvement of the super anion radicals in the autoxidation of pyrogollol and a convenient assay for superoxide dismutase,”
  36. (2010). L´ opez et al., “Role of oxidative stress and adenosine nucleotides in the liver of aging rats,”
  37. (2007). Le Couteur, “Basal activity of kupffer cells increases with old age,”
  38. (2002). M.C.Carrillo,S.Kanai,K.Miyasaka,andK.Kitani,“Aprotein free diet uncovers the potential age-difference in the hepatic detoxifying system, glutathione S-transferase,
  39. (2007). Mechanisms of aging and liver functions,”
  40. (2002). Membrane association of glutathione S-transferase mGSTA4-4, an enzyme that metabolizes lipid peroxidation products,”
  41. (2010). Modulating expression of brain heat shock proteins by estrogen in ovariectomized mice model of aging,”
  42. Na+,K +-ATPase and Mg2+-ATPase activities in different regions of rat brain during alloxan diabetes,”
  43. (2010). Nitration of specific tyrosines in FoF1 ATP synthase and activity loss in aging,”
  44. (2007). Protection of estrogens against the progression of chronic liver disease,”
  45. (2004). Protective effects of sodiumorthovanadate indiabetic reticulocytes andageing red blood cells of Wistar rats,”
  46. (2009). ra t t a gl i a n o ,L .B o n f ra t e ,C .V .D i o g o
  47. (2001). Relationship between age-related increases in rat liver lipid peroxidation and bile canalicular plasma membrane fluidity,”
  48. (2010). The effects of 17β-estradiol and vitamin e treatments on oxidative stress and antioxidant levels
  49. (2010). Therapeutic potential of Ganoderma
  50. (2007). Tresguerres, “Effect of chronic treatments with GH, melatonin, estrogens, and phytoestrogens on oxidative stress parameters in liver from aged female rats,”
  51. (2011). u m a r ,R .K .K a l e ,P .M c L e a n ,a n dN .Z .B a q u e r ,“ P r o t e c t i v e effects of 17β estradiol on altered age related neuronal parameters in female rat brain,”
  52. (2010). Women live longer than men: understanding molecular mechanisms offers opportunities to intervene by using estrogenic compounds,” Antioxidants and Redox Signaling,