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Duplex PCR assay for the detection of avian adeno virus and chicken anemia virus prevalent in Pakistan

By Latif U Rehman, Bakht Sultan, Ijaz Ali, Muhammad A Bhatti, Sana U Khan, Khaliq U Zaman, Anila T Jahangiri, Najib U Khan, Aqib Iqbal, Jehan Bakht, Zahoor A Swati and Muti U Rehman

Abstract

Avian Adeno viruses and Chicken Anemia Viruses cause serious economic losses to the poultry industry of Pakistan each year. Timely and efficient diagnosis of the viruses is needed in order to practice prevention and control strategies. In the first part of this study, we investigated broilers, breeder and Layer stocks for morbidity and mortality rates due to AAV and CAV infections and any co-infections by examining signs and symptoms typical of their infestation or post mortem examination. In the second part of the study, we developed a duplex PCR assay for the detection of AAV and CAV which is capable to simultaneously detect both the viral types prevalent in Pakistan with high sensitivity and 100% specificity

Topics: Methodology
Publisher: BioMed Central
OAI identifier: oai:pubmedcentral.nih.gov:3184108
Provided by: PubMed Central

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