Article thumbnail

Duplex PCR assay for the detection of avian adeno virus and chicken anemia virus prevalent in Pakistan

By Latif U Rehman, Bakht Sultan, Ijaz Ali, Muhammad A Bhatti, Sana U Khan, Khaliq U Zaman, Anila T Jahangiri, Najib U Khan, Aqib Iqbal, Jehan Bakht, Zahoor A Swati and Muti U Rehman


Avian Adeno viruses and Chicken Anemia Viruses cause serious economic losses to the poultry industry of Pakistan each year. Timely and efficient diagnosis of the viruses is needed in order to practice prevention and control strategies. In the first part of this study, we investigated broilers, breeder and Layer stocks for morbidity and mortality rates due to AAV and CAV infections and any co-infections by examining signs and symptoms typical of their infestation or post mortem examination. In the second part of the study, we developed a duplex PCR assay for the detection of AAV and CAV which is capable to simultaneously detect both the viral types prevalent in Pakistan with high sensitivity and 100% specificity

Topics: Methodology
Publisher: BioMed Central
OAI identifier:
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles


  1. (1950). A respiratory disease (bronchitis) of quail caused by a virus.
  2. (1949). A: The isolation in eggs of a new filterable agent which may be the cause of bovine lumpy skin disease.
  3. (1997). Barnes HJ: Characterization of an avian adenovirus associated with inclusion body hepatitis in day-old turkeys. Avian Dis
  4. (1976). BM: Studies on the antigenic relationship between an isolate (127) from the egg drop syndrome
  5. (1984). Characterization of adeno-virus isolated from geese. Avian Pathol
  6. (1957). DE: Observations on a chicken embryo lethal orphan (CELO) virus.
  7. (1999). Epidemiological studies on fowl adenoviruses isolated from cases of infectious hydropericardium. Avian Pathol
  8. (1960). Fontes AK: Virus isolated from avian lymphomas unrelated to lymphomatosis virus.
  9. (1996). Naeem K: In vitro and in vivo evaluation of avian adenovirus isolates from out-breaks of hydro pericardium syndrome.
  10. (1967). Pomeroy BS: Electron microscopic studies of quail bronchitis virus.
  11. (1964). Tsuba-hara H: Avian adenovirus: its properties and serological classification. Natl Inst Anim Health Q (Tokyo)
  12. (1980). WB: Incidence and distribution of avian adeno-virus group II splenomegaly of chickens. Avian Dis