Article thumbnail

Co-Interactive DNA-Binding between a Novel, Immunophilin-Like Shrimp Protein and VP15 Nucleocapsid Protein of White Spot Syndrome Virus

By Pakkakul Sangsuriya, Saengchan Senapin, Wei-Pang Huang, Chu-Fang Lo and Timothy W. Flegel

Abstract

White spot syndrome virus (WSSV) is one of the most serious pathogens of penaeid shrimp. Although its genome has been completely characterized, the functions of most of its putative proteins are not yet known. It has been suggested that the major nucleocapsid protein VP15 is involved in packaging of the WSSV genome during virion formation. However, little is known in its relationship with shrimp host cells. Using the yeast two-hybrid approach to screen a shrimp lymphoid organ (LO) cDNA library for proteins that might interact with VP15, a protein named PmFKBP46 was identified. It had high sequence similarity to a 46 kDa-immunophilin called FKBP46 from the lepidopteran Spodoptera frugiperda (the fall armyworm). The full length PmFKBP46 consisted of a 1,257-nucleotide open reading frame with a deduced amino acid sequence of 418 residues containing a putative FKBP-PPIase domain in the C-terminal region. Results from a GST pull-down assay and histological co-localization revealed that VP15 physically interacted with PmFKBP46 and that both proteins shared the same subcellular location in the nucleus. An electrophoretic mobility shift assay indicated that PmFKBP46 possessed DNA-binding activity and functionally co-interacted with VP15 in DNA binding. The overall results suggested that host PmFKBP46 might be involved in genome packaging by viral VP15 during virion assembly

Topics: Research Article
Publisher: Public Library of Science
OAI identifier: oai:pubmedcentral.nih.gov:3183051
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles

Citations

  1. (1996). A Handbook of Shrimp Pathology and Diagnostic Procedures for Diseases of Cultured Penaeid Shrimp. World Aquatic Society,
  2. (1997). A nonnatural transcriptional coactivator.
  3. (2004). A nuclear FK506-binding protein is a histone chaperone regulating rDNA silencing.
  4. (1992). A rapamycin-selective 25-kDa immunophilin.
  5. (1989). A receptor for the immunosuppressant FK506 is a cis-trans peptidyl-prolyl isomerase.
  6. (2007). b-integrin mediates WSSV infection.
  7. (2007). Characterization of a shrimp serine protease homolog, a binding protein of yellow head virus.
  8. (2007). Characterization of immunophilins in the silkmoth Bombyx mori.
  9. (2001). Complete genome sequence of the shrimp White spot bacilliform virus.
  10. (1995). Cyclophilin A and FKBP12 interact with YY1 and alter its transcriptional activity.
  11. (2010). DNA condensates organized by the capsid protein VP15 in white spot syndrome virus.
  12. (1989). FK-506, a potent novel immunosuppressive agent, binds to a cytosolic protein which is distinct from the cyclosporine A-binding protein, cyclophilin.
  13. (1994). FKBP46, a novel Sf9 insect cell nuclear immunophilin that forms a protein-kinase complex.
  14. (2011). FKBPs in chromatin modification and cancer.
  15. (2005). Four viral proteins of white spot syndrome virus (WSSV) that attach to shrimp cell membranes.
  16. (1993). Galat A
  17. (2010). Genome-wide analysis of genes encoding FK506-binding proteins in rice.
  18. (2006). Hepatitis C virus RNA replication is regulated by FKBP8 and Hsp90.
  19. (2010). I-TASSER: a unified platform for automated protein structure and function prediction.
  20. (2009). Identification and characterization of nuclear localization signals within the nucleocapsid protein VP15 of white spot syndrome virus.
  21. (2004). Identification of a novel shrimp protein phosphatase and its association with latencey-related ORF427 of white spot syndrome virus.
  22. (2006). Identification of the nucleocapsid, tegument, and envelope proteins of the shrimp white spot syndrome virus virion.
  23. (2002). Identification of VP19 and VP15 of white spot syndrome virus (WSSV) and glycosylation status of the WSSV major structural proteins.
  24. (2006). Immunophilins: for the love of proteins.
  25. (2003). Inherent calcineurin inhibitor FKBP38 targets Bcl-2 to mitochondria and inhibits apoptosis.
  26. (2005). Molecular characterization of FK506 binding protein 38 and its potential regulatory role on the anti-apoptotic protein Bcl-2.
  27. (2005). Nucleocapsid protein VP15 is the basic DNA binding protein of white spot syndrome virus of shrimp.
  28. (2009). Penaeus monodon chitin-binding protein (PmCBP) is involved in white spot syndrome virus (WSSV) infection.
  29. (1993). Peptidylproline cis-trans-isomerases: immunophilins.
  30. (2009). Phongdara A
  31. (2005). Plunder and stowaways: incorporation of cellular proteins by enveloped viruses.
  32. (2006). PmRab7 is a VP28-binding protein involved in white spot syndrome virus infection in shrimp.
  33. (2004). Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequence Proteomics
  34. (1990). Probing immunosuppressant action with a nonnatural immunophilin ligand.
  35. (2010). PROSITE, a protein domain database for functional characterization and annotation.
  36. (2002). Proteomic analysis of shrimp white spot syndrome viral proteins and characterization of a novel envelope protein VP466.
  37. (2000). Rapid and reliable protein extraction from yeast.
  38. (2005). RING-H2 protein WSSV249 from white spot syndrome virus sequesters a shrimp ubiquitinconjugating enzyme, PvUbc, for viral pathogenesis.
  39. (1983). Separation of the haemocyte populations of Carcinus maenas and other marine decapods, and prophenoloxidase distribution.
  40. (2011). Shrimp molecular responses to viral pathogens.
  41. (1995). Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure.
  42. (2002). The involvement of mammalian and plant FK506-binding proteins (FKBPs) in development.
  43. (2001). The structure and function of a gene encoding a basic peptide from prawn white spot syndrome virus.
  44. (2001). The white spot syndrome virus DNA genome sequence.
  45. (1991). Two interdependent basic domains in nucleoplsmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequences.
  46. (2007). White spot syndrome virus envelope protein VP53A interacts with Penaeus monodon chitin-binding protein (PmCBP).
  47. (2008). White spot syndrome virus protein ICP11: A histone-binding DNA mimic that disrupts nucleosome assembly.
  48. (2010). White spot syndrome virus: an overview on an emergent concern.