Article thumbnail

Effect of Novel Marine Nutraceuticals on IL-1α-Mediated TNF-α Release from UVB-Irradiated Human Melanocyte-Derived Cells

By Visalini Muthusamy, Lynn D. Hodges, Theodore A. Macrides, Glen M. Boyle and Terrence J. Piva

Abstract

UV-induced inflammation and reactive oxygen species formation are involved in the development of melanoma. Natural products like 5β-scymnol and CO2-supercritical fluid extract (CO2-SFE) of mussel oil contain anti-inflammatory and antioxidant properties that may aid in reducing the deleterious effects of UV radiation. Therefore, their effect on the release of the proinflammatory cytokine, tumour necrosis factor-α (TNF-α), from UVB-irradiated human melanocytic cells was examined. Human epidermal melanocytes (HEM) and MM96L melanoma cells were exposed to UVB radiation and IL-1α. Cell viability and TNF-α levels were determined 24 hours after-irradiation while p38 mitogen-activated protein kinase (MAPK) activation was observed at 15 min after-irradiation. When α-tocopherol, CO2-SFE mussel oil, and 5β-scymnol were added to the UVB-irradiated HEM cells treated with IL-1α, TNF-α levels fell by 53%, 65%, and 76%, respectively, while no inhibition was evident in MM96L cells. This effect was not due to inhibition of the intracellular p38 MAPK signalling pathway. These compounds may be useful in preventing inflammation-induced damage to normal melanocytes

Topics: Research Article
Publisher: Hindawi Publishing Corporation
OAI identifier: oai:pubmedcentral.nih.gov:3179886
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles

Citations

  1. (2009). A .J .S c h e t t e r ,N .H .H e e g a a r d ,a n dC .C .H a r r i s ,“ I n fl a m -mation and cancer: Interweaving microRNA, free radical, cytokine and p53 pathways,”
  2. (2007). A .P .T r e s c h o w ,L .D .H o d g e s
  3. (1997). A comparison of the hydroxyl radical scavenging properties of the shark bile steroid 5β-scymnol and plant pycnogenols,”
  4. (2008). All-trans retinoic acid attenuates ultraviolet radiation-induced down-regulation of aquaporin-3 and water permeability in human keratinocytes,”
  5. (2003). An investigation of the hepatoprotective mechanism of the shark bile sterol, scymnol,
  6. (2008). Anthocyanins from black soybean seed coats inhibit UVB-induced inflammatory cylooxygenase-2 gene expression and PGE2 production through regulation of the nuclear factor-κB and phosphatidylinositol 3-kinase/Akt pathway,”
  7. (2000). Anti-inflammatory effects of a stabilized lipid extract of Perna canaliculus
  8. (2007). Anticyclooxygenase effects of lipid extracts from the New Zealand green-lipped mussel,
  9. (1990). Antioxidant activity of indapamide and its metabolite,”
  10. (2009). B a s h i r ,M .R .S h a r m a ,a n dV .P .W e r t h ,“ U V Ba n d proinflammatory cytokines synergistically activate TNF-α productioninkeratinocytesthroughenhancedgenetranscription,”
  11. (1997). D.R.Haynes,andJ.Broadbent,“Anti-inflammatoryactivityof a lipid fraction (Lyprinol) from the NZ green-lipped mussel,”
  12. (2007). Dietary grape seed proanthocyanidins inhibit UVB-induced oxidative stress and activation of mitogen-activated protein kinases and nuclear factor-κB signaling in
  13. (1997). Differences in responses of interleukin-1 and tumor necrosis factor α production and secretion to cyclosporin-A and ultraviolet B-irradiation by normal and transformed keratinocytes cultures,”
  14. (2002). Differential role of hydrogen peroxide in UV-induced signal transduction,”
  15. (1999). Down-regulation of tumor necrosis factor α expression by activating transcription factor 2 increases UVC-induced apoptosis of late-stage melanoma cells,”
  16. (2009). Dynamic expression of epidermal caspase 8 simulates a wound healing response,”
  17. (2009). e n g ,X .L u o ,L .M a ,K .K a n g ,a n dZ .Z h e n g ,“ R e v e r s a lo f ultraviolet B-induced immunosuppression by inhibition of the extracellular signal-regulated mitogen-activated protein kinase,”
  18. (2000). Effects of topical and oral vitamin E on pigmentation and skin cancer induced by ultraviolet irradiation
  19. (2009). Engin, “Alpha-tocopherol: looking beyond an antioxidant,”
  20. (2001). Expression of ring finger-deleted TRAF2 sensitizes metastatic melanoma cells to apoptosis via up-regulation of p38, TNFα and suppression of NF-κB activities,”
  21. (2007). G r a y - S c h o p f e r ,M .K a r a s a r i d e s ,R .H a y w a r d ,a n dR . Marais, “Tumor necrosis factor-α blocks apoptosis in melanoma cells when BRAF signaling is inhibited,”
  22. (2006). Grape seed proanthocyanidins inhibit UV-radiation-induced oxidative stress and activation of MAPK and NF-κB signaling in human epidermal keratinocytes,”
  23. (2009). H u y n h ,K .S .C h a n ,a n dT .J .P i v a ,“ E ffect of ultraviolet radiationontheexpressionofpp38MAPKandfurininhuman keratinocyte-derived cell lines,”
  24. (1990). How to characterize a biological antioxidant,”
  25. (1979). Human malignant melanoma cell lines,”
  26. (2003). IL-12 completely blocks ultraviolet-induced secretion of tumor necrosis factor α from cultured skin fibroblasts and keratinocytes,”
  27. (2003). Induction of tumor necrosis Factor-α by UVB: a role for reactive oxygen intermediates and eicosanoids,”
  28. (2005). Inflammation, gene mutation and photoimmunosuppression in response to UVR-induced oxidative damage contributes to photocarcinogenesis,”
  29. (1997). Inhibition of cyclobutane pyrimidine dimer formation in epidermal p53 gene of UV-irradiated mice by α-tocopherol,”
  30. (2010). Interleukins 1α and 1β secreted by some melanoma cell lines strongly reduce expression of MITF-M and melanocyte differentiation antigens,”
  31. (2008). Involvement of ROS/ASMase/JNK signalling pathway in inhibiting UVA-induced apoptosis of HaCaT cells by polypeptide from
  32. (2008). JNK mediates UVB-induced apoptosis upstream lysosomal membrane permeabilization and Bcl-2familyproteins,”Apoptosis,vol.13,no.9,pp.1111–1120,
  33. (2009). Keratinocyte growth factor down-regulates intracellular ROS production induced by UVB,”
  34. (2002). Lipid, FA, and sterol composition of New Zealand g r e e nl i p p e dm u s s e l( Perna canaliculus) and Tasmanian blue mussel (Mytilus edulis),” Lipids,
  35. (2008). Methodological aspects about in vitro evaluation of antioxidant properties,”
  36. (2000). Okuhama et al., “Cat’s claw inhibits TNFα production and scavenges free radicals: role in cytoprotection,”
  37. (1993). On the synthesis of scymnol,”
  38. (2000). Photoprotective action of topically applied vitamin
  39. (2008). Polymorphisms in the interleukin-1 gene influence the stratum corneum interleukin-1α concentration in uninvolved skin of patients with chronic irritant contactdermatitis,”ContactDermatitis,vol.58,no.5,pp.263– 268,
  40. (2009). Protective effect of ferulic acid ethyl ester against oxidative stress mediated by UVB irradiation in human epidermal melanocytes,”
  41. (2004). S.McPhee,“Anti-inflammatorypropertiesoftheNewZealand green-lipped mussel, Perna canaliculus,”
  42. (1989). Scymnol sulphate salts isolated from shark tissues for the treatment of liver and skin ailments,” (Patent:
  43. (2009). Sensitization of melanoma cells to TRAIL by UVB-induced and NF-κB-mediated downregulation of xIAP,”
  44. (2006). Sunscreens with an absorption maximum of ≥360nm provide optimal protection against UVA1-induced expression of matrix
  45. (2000). Super-critical lipid extract from mussels having anti-inflammatory activity,” United States Patent 6083536,
  46. (2007). T.IshidaandI.Sakaguchi,“Protectionofhumankeratinocytes from UVB-induced inflammation using root extract of Lithospermum erythrorhizon,”
  47. (2003). Takayasu et al., “Cancer chemopreventive effects of oral feeding α-tocopherol on ultraviolet light B induced photocarcinogenesis of hairless mouse,”
  48. (2008). The CO2-SFE crude lipid extract and the free fatty acid extract from Perna canaliculus have anti-inflammatory effects on adjuvantinduced arthritis
  49. (2010). The StrataTest human skin model, a consistent in vitro alternative for toxicological testing,” Toxicology
  50. (1998). Topical application of α-tocopherol modulates the antioxidant network and diminishes ultraviolet-induced oxidative damage in murine skin,”
  51. (2010). u t h u s a m ya n dT .J .P i v a ,“ T h eU Vr e s p o n s eo ft h es k i n : a review of the MAPK, NFκB and TNFα signal transduction pathways,”
  52. (2006). Ultraviolet (UV) A- and UVB-induced redox alterations and activation of nuclear factor-κB in human melanocytes-protective effects ofOxidative
  53. (2002). Ultraviolet A augments solar-simulated ultraviolet radiationinducedlocalsuppressionofrecallresponsesinhumans,”JournalofInvestigativeDermatology,vol.118,no.6,pp.1032–1037,
  54. (2001). Up-regulation of p21 WAF1/CIP1 by histone deacetylase inhibitors reduces their cytotoxicity,”
  55. (1998). UV-irradiation depletes antioxidants and causes oxidative damage in a model of human skin,”
  56. (1999). v a ma n dR .M .T y r r e l l ,“ T h er o l eo fm e l a n i ni nt h e induction of oxidative DNA base damage by ultraviolet A irradiation of DNA or melanoma cells,”