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Phage Displayed Peptides to Avian H5N1 Virus Distinguished the Virus from Other Viruses

By Dan Wu, Guangxing Li, Chengfeng Qin and Xiaofeng Ren


The purpose of the current study was to identify potential ligands and develop a novel diagnostic test to highly pathogenic avian influenza A virus (HPAI), subtype H5N1 viruses using phage display technology. The H5N1 viruses were used as an immobilized target in a biopanning process using a 12-mer phage display random peptide library. After five rounds of panning, three phages expressing peptides HAWDPIPARDPF, AAWHLIVALAPN or ATSHLHVRLPSK had a specific binding activity to H5N1 viruses were isolated. Putative binding motifs to H5N1 viruses were identified by DNA sequencing. In terms of the minimum quantity of viruses, the phage-based ELISA was better than antiserum-based ELISA and a manual, semi-quantitative endpoint RT-PCR for detecting H5N1 viruses. More importantly, the selected phages bearing the specific peptides to H5N1 viruses were capable of differentiating this virus from other avian viruses in enzyme-linked immunosorbent assays

Topics: Research Article
Publisher: Public Library of Science
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Provided by: PubMed Central

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  10. (2007). Efficacy of oseltamivir therapy in ferrets inoculated with different clades of H5N1 influenza virus.
  11. (1998). From peptides to drugs via phage display.
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  13. (2004). Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia.
  14. Govorkova EA (2006) H5N1 influenza–continuing evolution and spread.
  15. (1998). Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus.
  16. (2008). Identification of a peptide sequence that improves transport of macromolecules across the intestinal mucosal barrier targeting goblet cells.
  17. (2009). Inhibition of influenza virus infections by sialylgalactose-binding peptides selected from a phage library.
  18. (2010). Multiplexed, rapid detection of H5N1 using a PCR-free nanoparticle-based genomic microarray assay.
  19. (2008). Neutralizing human monoclonal antibody against H5N1 influenza HA selected from a Fabphage display library.
  20. (2001). Novel vaccine strategies to T-independent antigens.
  21. (2009). Organization. Epidemic and pandemic alert and response (EPR): avian influenza—situation in China—update [cited
  22. (2005). Osterhaus A
  23. (2011). Phage displayed peptides recognizing porcine aminopeptidase N inhibit transmissible gastroenteritis coronavirus infection in vitro.
  24. (2010). Phages harboring specific peptides to N protein of PRRSV distinguish the virus from other viruses.
  25. (2006). Qinghai-like A (H5N1) from domestic cats, northern Iraq. Emerging Infectious Diseases
  26. (1990). Searching for peptide ligands with an epitope library.