Article thumbnail

Hematological and Serum Biochemical Analyses in Experimental Caprine Besnoitiosis

By Saeed Nazifi, Ahmad Oryan and Fatemeh Namazi


This study was undertaken to investigate the hematological and biochemical changes in experimentally infected goats with Besnoitia caprae from the time of infection till 360 days post-infection (PI). Six male goats were inoculated subcutaneously with 13×107 bradyzoites of B. caprae, and blood samples were collected from the jugular vein. The total erythrocyte and total leukocyte counts, hematocrit value, and differential leukocyte counts were determined. Serum biochemical analysis, including the total protein, albumin, total globulin, cholesterol, triglyceride, chloride, testosterone, calcium (Ca2+), inorganic phosphorus, sodium (Na+), potassium (K+), iron (Fe2+), glucose, serum amyloid A (SAA), haptoglobin (Hp), fibrinogen, ceruloplasmin, aspartate aminotransferase, alanine aminotransferase, creatine kinase, lactate dehydrogenase, and alkaline phosphatase, was undertaken. Skin biopsy from the limbs were collected at weekly intervals and histologically examined for Besnoitia cysts. Cysts were present in the skin biopsies of the leg of the infected goats from day 28 PI. There were variations in hematological analyses, but no significant difference was seen. From day 30 to 360 PI, results showed that SAA, Hp, fibrinogen, and ceruloplasmin concentrations increased, whereas testosterone concentrations decreased. Infected goats exhibited decrease of albumin and increase of serum total protein and globulin concentrations. By contrast, there were no significant differences in the remained analyses concentrations

Topics: Original Article
Publisher: The Korean Society for Parasitology
OAI identifier:
Provided by: PubMed Central

Suggested articles


  1. (1983). A peroxidasecoupled method for the colorimetric determination of serum triglycerides. Clin Chem
  2. (1993). A review of besnoitiosis with special reference to goats. Zimbabwe Vet J
  3. (2005). Acute phase reaction and acute phase proteins.
  4. (1989). An epidemic of besnoitiosis in goats in Kenya. Vet Rec
  5. (1997). An epizootic of besnoitiosis in goats in Fars province of Iran. Vet Res Commun
  6. (2003). Besnoitia oryctofelisi n. sp. (Protozoa: Apicomplexa) from domestic rabbits. Parasitology
  7. (1979). Besnoitiosis in wild and domestic goats in Iran. Cornell Vet
  8. (2008). Besnoitiosis of the reproductive tract of male goats. Comp Clin Path
  9. (2007). Characterisation of the pig acute phase protein response to road transport.
  10. (2004). Comparative aspects of blood coagulation.
  11. (1994). Concentrations of serum amyloid A (SAA) and haptoglobin (Hp) as parameters of inflammatory diseases in cattle. Vet Q
  12. (2004). Current research on acute phase proteins in veterinary diagnosis: an overview.
  13. (1999). Detection of haptoglobin in the highdensity lipoprotein and the very high-density lipoprotein fractions from sera of calves with experimental pneumonia and cows with naturally occurring fatty liver.
  14. (2004). Detection of Neospora caninum in an aborted goat fetus. Vet Parasitol
  15. (2003). Effect of transportation and commingling on the acute-phase protein response, growth, and feed intake of newly weaned beef calves.
  16. (1998). endocrine, immune, and performance measures for pigs exposed to acute stress.
  17. (1993). Essentials of Veterinary Hematology,
  18. (2002). Evaluation of hematological parameters in caprine besnoitiosis.
  19. (2008). Fundamentals of Veterinary Clinical Pathology. 2nd ed.
  20. (1995). Influence of physical stress on the plasma concentrations of serum amyloid A (SAA) and haptoglobin (Hp) in calves. Vet Q
  21. (1970). Measurement of human serum ceruloplasmin by its p-phenylenediamine oxidase activity. Clin Chem
  22. (2000). Molecular phylogeny of Besnoitia and the genetic relationships among Besnoitia of cattle, wildebeest and goats. Protist
  23. (2008). Pathology and serum biochemical changes in natural caprine besnoitiosis. Rev Med Vet (Toulouse)
  24. (2007). Pathology of testis and epididymis in native goats in southern Iran. Comp Clin Path
  25. (2000). Recent advances and future prospects for the use of acute phase proteins as markers of disease in animals. Rev Med Vet (Toulouse)
  26. (2000). Schalm’s Veterinary Hematology.
  27. (1999). Semen characteristics of goats with subacute, acute and chromic besnoitiosis.
  28. (1999). Serum amyloid A, the major vertebrate acute-phase reactant.
  29. (1997). Serum proteins and dysproteinaemias.
  30. (2011). Stress and acute phase protein response: an inconspi-138
  31. (2003). The acute phase response in calves experimentally infected with bovine viral diarrhea virus and/or Mannheimia haemolytica.
  32. (2003). The effect of abrupt weaning of suckler calves on the plasma concentrations of cortisol, catecholamines, leukocytes, acute phase proteins and in vitro interferon gamma production.
  33. (2003). The protozoan parasite Theileria annulata induces a distinct acute phase protein response in cattle that is associated with pathology.
  34. (2004). The time is right for acute phase protein assays.
  35. (2008). Ultrastructure and pathology of Besnoitia caprae in the naturally infected goats of Kerman, East of Iran. Parasitol Res
  36. (2004). Veterinary Hematology and Clinical Chemistry.

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.