Article thumbnail

Intron retention in the Drosophila melanogaster Rieske iron sulphur protein gene generated a new protein

By Alisson M. Gontijo, Veronica Miguela, Michael F. Whiting, R.C. Woodruff and Maria Dominguez


Genomes can encode a variety of proteins with unrelated architectures and activities. It is known that protein-coding genes of de novo origin have significantly contributed to this diversity. However, the molecular mechanisms and evolutionary processes behind these originations are still poorly understood. Here we show that the last 102 codons of a novel gene, Noble, assembled directly from non-coding DNA following an intronic deletion that induced alternative intron retention at the Drosophila melanogaster Rieske Iron Sulphur Protein (RFeSP) locus. A systematic analysis of the evolutionary processes behind the origin of Noble showed that its emergence was strongly biased by natural selection on and around the RFeSP locus. Noble mRNA is shown to encode a bona fide protein that lacks an iron sulphur domain and localizes to mitochondria. Together, these results demonstrate the generation of a novel protein at a naturally selected site

Topics: Article
Publisher: Nature Publishing Group
OAI identifier:
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles


  1. (2003). A Balbiani body and the fusome mediate mitochondrial inheritance during Drosophila oogenesis.
  2. (2002). A Drosophila full-length cDNA resource.
  3. (2007). A phylogeny of Drosophilidae using the amyrel gene: questioning the Drosophila melanogaster species group boundaries.
  4. (2008). African Drosophila melanogaster and D. simulans populations have similar levels of sequence variability, suggesting comparable effective population sizes.
  5. (1984). Birth of a unique enzyme from an alternative reading frame of the preexisted, internally repetitious coding sequence.
  6. (2010). Drosophila melanogaster recombination rate calculator. Gene 463,
  7. (2004). Duplication and divergence: the evolution of new genes and old ideas.
  8. (1995). Efficiencies of different genes and different tree-building methods in recovering a known vertebrate phylogeny.
  9. (2003). Evolution of alternative splicing: deletions, insertions and origin of functional parts of proteins from intron sequences.
  10. (2005). Evolution of the Insects
  11. (2000). Evolutionary novelties in islands: Drosophila santomea, a new melanogaster sister species from São Tomé.
  12. (2002). Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster euchromatic genome sequence.
  13. (2009). Frequency spectrum neutrality tests: one for all and all for one.
  14. (2006). Frequent appearance of novel protein-coding sequences by frameshift translation.
  15. (2005). Functional divergence of proteins through frameshift mutations.
  16. (2006). Functions of the nonsense-mediated mRNA decay pathway in Drosophila development.
  17. (2005). Gene duplication and the origin of novel proteins.
  18. (1988). Genetic variation of Drosophila melanogaster natural populations.
  19. (2009). Genome-wide identification of alternative splice forms down-regulated by nonsense-mediated mRNA decay in Drosophila.
  20. (2007). Genomes Consortium. Evolution of genes and genomes on the Drosophila phylogeny.
  21. (2003). Genomic and structural aspects of protein evolution.
  22. (2000). HIV Signature and Sequence Variation Analysis.
  23. (2008). Improving the photostability of bright monomeric orange and red fluorescent proteins.
  24. in: The Evolutionary Biology of Flies (eds
  25. (2006). Intron length evolution in Drosophila.
  26. (2007). Investigations of combinations of mutations in the jellyfish green fluorescent protein (GFP) that afford brighter fluorescence, and use of a version (VisGreen) in plant, bacterial, and animal cells.
  27. (2000). Molecular Evolution and Phylogenetics
  28. (2003). Nonsense-mediated mRNA decay in Drosophila: at the intersection of the yeast and mammalian pathways.
  29. (2009). Nucleotide and copy-number polymorphism at the odorant receptor genes Or22a and Or22b in Drosophila melanogaster.
  30. (1974). On some principles governing molecular evolution.
  31. (2010). Origins, evolution, and phenotypic impact of new genes.
  32. (2009). Overlapping genes produce proteins with unusual sequence properties and offer insight into de novo protein creation.
  33. (2008). Phylogenetics and a timescale for diversification of the earliest true flies (Insecta: Diptera) based on multiple nuclear genes.
  34. (2007). Quality control of eukaryotic mRNA: safeguarding cells from abnormal mRNA function.
  35. (2010). Ribosomal protein genes of holometabolan insects reject the Halteria, instead revealing a close affinity of Strepsiptera with Coleoptera.
  36. (2009). Single-copy nuclear genes resolve the phylogeny of the holometabolous insects.
  37. (1992). Splicing signals in Drosophila: intron size, information content, and consensus sequences.
  38. (2004). Temporal patterns of fruit fly (Drosophila) evolution revealed by mutation clocks.
  39. (2007). The (in)dependence of alternative splicing and gene duplication.
  40. (1999). The Berkeley Drosophila Genome Project gene disruption project: single P-element insertions mutating 25% of vital Drosophila genes.
  41. (2002). The Drosophila gene collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes.
  42. (2010). The evolution of gene duplications: classifying and distinguishing between models.
  43. (2000). The evolutionary fate and consequences of duplicate genes.
  44. (2007). The frailty of adaptive hypotheses for the origins of organismal complexity.
  45. (2000). The genome sequence of Drosophila melanogaster.
  46. (2010). The neutral theory of molecular evolution in the genomic era.
  47. (2003). The origin of new genes: glimpses from the young and old.
  48. (2008). The population genetics of dN/dS.
  49. (1997). The Strepsiptera problem: phylogeny of the holometabolous insect orders inferred from 18S and 28S ribosomal DNA sequences and morphology.
  50. (2004). Three new Drosophila markers of intracellular membranes.
  51. (1997). Tumor suppression at the mouse INK4a locus mediated by the alternative reading frame product p19ARF.
  52. (1999). Unusual haplotype structure at the proximal breakpoint of In(2L)t in a natural population of Drosophila melanogaster.