Article thumbnail

Hepatoprotective Effects of Orthosiphon stamineus Extract on Thioacetamide-Induced Liver Cirrhosis in Rats

By Mohammed A. Alshawsh, Mahmood Ameen Abdulla, Salmah Ismail and Zahra A. Amin

Abstract

Orthosiphon stamineus as medicinal plant is commonly used in Malaysia for treatment of hepatitis and jaundice; in this study, the ethanol extracts were applied to evaluate the hepatoprotective effects in a thioacetamide-induced hepatotoxic model in Sprague Dawley rats. Five groups of adult rats were arranged as follows: Group 1 (normal control group), Group 2 Thioacetamide (TAA) as positive control (hepatotoxic group), Group 3 Silymarin as a well-known standard drug (hepatoprotective group), and Groups 4 and 5 as high and low dose (treatment groups). After 60-day treatment, all rats were sacrificed. The hepatotoxic group showed a coarse granulation on the liver surface when compared to the smooth aspect observed on the liver surface of the other groups. Histopathological study confirmed the result; moreover, there was a significant increase in serum liver biochemical parameters (ALT, AST, ALP, and Bilirubin) and the level of liver Malondialdehyde (MDA), accompanied by a significant decrease in the level of total protein and Albumin in the TAA control group when compared with that of the normal group. The high-dose treatment group (200 mg/kg) significantly restored the elevated liver function enzymes near to normal. This study revealed that 200 mg/kg extracts of O. stamineus exerted a hepatoprotective effect

Topics: Research Article
Publisher: Hindawi Publishing Corporation
OAI identifier: oai:pubmedcentral.nih.gov:3106356
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles

Citations

  1. (1996). a r a s w a t ,P .K .S .V i s e n ,R .D a y a l ,D .P .A g a r w a l ,a n d
  2. (2008). An investigation of the anti-inflammatory and analgesic effects of Orthosiphon stamineus leaf extract,”
  3. Association of Official Analytical Chemists, Official methodsof analysis ofthe Associationofthe Analytical Chemists,
  4. (1987). Biochemical and morphological studies on production and regression of experimental liver cirrhosis induced by thioacetamide
  5. (2008). C h i n ,H .H .A b a s ,a n dI .S a b a r i a h ,“ T o x i c i t ys t u d y of orthosiphon stamineus benth (misai kucing) on Sprague dawley rats,”
  6. (2007). Effects of Orthosiphon stamineus aqueous extract on plasma glucose concentration and lipid profile in normal and streptozotocin-induced diabetic rats,”
  7. (2002). Evaluation of hepatoprotective potential of jigrine post-treatment against thioacetamide induced hepatic damage,”
  8. (2000). F.Sun,S.Hayami,Y.Ogirietal.,“Evaluationofoxidativestress based onlipidhydroperoxide, vitaminCandvitaminEduring apoptosis and necrosis caused by thioacetamide in rat liver,”
  9. (2000). Flavonols, flavones and flavanols—nature, occurrence and dietary burden,”
  10. (2008). h i n ,G .A k o w u a h ,A .H u s s i ne ta l . ,Toxicity and In-vivo Antioxidant Effect of Orthosiphon stamineus Leaf Extracts in Rats,
  11. (2008). Hepatoprotective activity of Silybum marianum and Cichorium intybus againstthioacetamide in rat,”
  12. (2007). Herbal medicine in the treatment of liver diseases,”
  13. (2000). K.Ohashi,T.Bohgaki,andH.Shibuya,“Antihypertensive substance in the leaves of kumis kucing (Orthosiphon aristatus)
  14. (2010). Koc ¸ak-Toker,“Effect ofcarnosine against thioacetamide-induced liver cirrhosis in rat,” Peptides,
  15. (2000). Medicinal plants with hepatoprotective properties,”
  16. (2003). O l a h ,L .R a d u ,C .M o g o s
  17. Orthosiphonstamineus Benth,” in Medicinal plants of Southeast Asia,
  18. (1999). r e f t ,F .D o m b r o w s k i ,W .B l o c k ,R .B a c h m a n n ,U .P f e i f e r
  19. (2004). Radical scavenging activity of methanol leaf extracts
  20. (1996). Serum amino acid changes in rats with thioacetamide-induced liver cirrhosis,”
  21. (1959). Spectrophotometric determination of quercetin,”
  22. (2008). Studies on diuretic and hypouricemic effects of Orthosiphon stamineus methanol extracts in rats,”
  23. (2005). The effects of different extraction solvents of varying polarities on polyphenols of Orthosiphon stamineus and evaluation of the free radical-scavenging activity,”
  24. The Green Pharmacy of Malaysia, Vinpress Sdn Bhd,
  25. (1991). u m a r y o n o ,P .P r o k s c h ,V .W r a y ,L .W i t t e ,a n dT .H a r t -mann, “Qualitative and quantitative analysis of the phenolic constituents from Orthosiphon aristatus,” Planta Medica,v o l .
  26. (2010). valuation ofselected Malaysianmedicinalplants onphaseI drug metabolizing enzymes,
  27. (2009). Z.Asmawi,“Evaluation ofthe anti-pyretic potential of Orthosiphon stamineus Benth standardized extract,”