Article thumbnail

Annexin-A5 assembled into two-dimensional arrays promotes cell membrane repair

By Anthony Bouter, Céline Gounou, Rémi Bérat, Sisareuth Tan, Bernard Gallois, Thierry Granier, Béatrice Langlois d'Estaintot, Ernst Pöschl, Bent Brachvogel and Alain R. Brisson


Eukaryotic cells possess a universal repair machinery that ensures rapid resealing of plasma membrane disruptions. Before resealing, the torn membrane is submitted to considerable tension, which functions to expand the disruption. Here we show that annexin-A5 (AnxA5), a protein that self-assembles into two-dimensional (2D) arrays on membranes upon Ca2+ activation, promotes membrane repair. Compared with wild-type mouse perivascular cells, AnxA5-null cells exhibit a severe membrane repair defect. Membrane repair in AnxA5-null cells is rescued by addition of AnxA5, which binds exclusively to disrupted membrane areas. In contrast, an AnxA5 mutant that lacks the ability of forming 2D arrays is unable to promote membrane repair. We propose that AnxA5 participates in a previously unrecognized step of the membrane repair process: triggered by the local influx of Ca2+, AnxA5 proteins bind to torn membrane edges and form a 2D array, which prevents wound expansion and promotes membrane resealing

Topics: Article
Publisher: Nature Publishing Group
OAI identifier:
Provided by: PubMed Central

To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.

Suggested articles


  1. (2000). A decrease in membrane tension precedes successful cell-membrane repair.
  2. (2005). An emergency response team for membrane repair.
  3. (2004). Annexin II regulates fibrin homeostasis and neoangiogenesis in vivo.
  4. (1994). Annexin structure and membrane interactions: a molecular perspective.
  5. (2008). Annexin-A1: a pivotal regulator of the innate and adaptive immune systems.
  6. (2005). Annexins: linking Ca2+ signalling to membrane dynamics.
  7. (2004). Antibody-mediated interference with annexins in the antiphospholipid syndrome.
  8. (1995). Calcium-regulated exocytosis is required for cell membrane resealing.
  9. (1994). Cell membrane resealing by a vesicular mechanism similar to neurotransmitter release.
  10. (1992). Clustering of lipid-bound annexin V may explain its anticoagulant effect.
  11. (2003). Defective membrane repair in dysferlin-deficient muscular dystrophy.
  12. (2004). Dysferlin and the plasma membrane repair in muscular dystrophy.
  13. (2007). Dysferlin in membrane trafficking and patch repair.
  14. (2003). Dysferlin interacts with annexins A1 and A2 and mediates sarcolemmal wound-healing.
  15. (1998). Dysferlin, a novel skeletal muscle gene, is mutated in Miyoshi myopathy and limb girdle muscular dystrophy.
  16. (2005). Dystrophic heart failure blocked by membrane sealant poloxamer.
  17. (1994). Formation of two-dimensional arrays of annexin V on phosphatidylserinecontaining liposomes.
  18. (1998). Growth of protein 2D crystals on supported planar lipid bilayers imaged in situ by AFM.
  19. (2009). Membrane repair defects in muscular dystrophy are linked to altered interaction between MG53, caveolin-3, and dysferlin.
  20. (2009). MG53 nucleates assembly of cell membrane repair machinery.
  21. (1996). Modulation of membrane dynamics and cell motility by membrane tension.
  22. (2005). On the kinetics of adsorption and two-dimensional self-assembly of annexin A5 on supported lipid bilayers.
  23. (2000). Patching plasma membrane disruptions with cytoplasmic membrane.
  24. (2001). Phosphorylation mutants elucidate the mechanism of annexin IV-mediated membrane aggregation.
  25. (2003). Plasma membrane disruption: repair, prevention, adaptation.
  26. Requirement for annexin A1 in plasma membrane repair.
  27. (1997). Structural analysis of junctions formed between lipid membranes and several annexins by cryoelectron microscopy.
  28. (1991). Sub-domain structure of lipid-bound annexin-V resolved by electron image analysis.
  29. (2004). The annexins.
  30. (1990). The crystal and molecular structure of human annexin V, an anticoagulant protein that binds to calcium and membranes.
  31. (2004). The molecular arrangement of membrane-bound annexin A2-S100A10 tetramer as revealed by scanning force microscopy.
  32. (1994). Three-dimensional structure of membrane-bound annexin V. A correlative electron microscopy-X-ray crystallography study.