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A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells

By Michael J Edel, Cristina Menchon, Jose Miguel Andres Vaquero and Juan Carlos Izpisua Belmonte

Abstract

Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSCs) present a great opportunity to treat and model human disease as a cell replacement therapy. There is a growing pressure to understand better the signal transduction pathways regulating pluripotency and self-renewal of these special cells in order to deliver a safe and reliable cell based therapy in the near future. Many signal transduction pathways converge on two major cell functions associated with self-renewal and pluripotency: control of the cell cycle and apoptosis, although a standard method is lacking across the field. Here we present a detailed protocol to assess the cell cycle and apoptosis of ESC and iPSCs as a single reference point offering an easy to use standard approach across the field

Topics: Methodology
Publisher: BioMed Central
OAI identifier: oai:pubmedcentral.nih.gov:3101157
Provided by: PubMed Central

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