Skip to main content
Article thumbnail
Location of Repository

Gene Expression Profiling in Lungs of Chronic Asthmatic Mice Treated with Galectin-3: Downregulation of Inflammatory and Regulatory Genes

By Esther López, M. Paz Zafra, Beatriz Sastre, Cristina Gámez, Carlos Lahoz and Victoria del Pozo


Background. Asthma is a disorder characterized by a predominance of Th2 cells and eosinophilic inflammation. Suppressors of cytokine signaling (SOCS) proteins act as negative regulators of cytokine signaling. In particular, SOCS1 and SOCS3 play an important role in immune response by controlling the balance between Th1 and Th2 cells. In a previous study, we demonstrated that treatment of chronic asthmatic mice with gene therapy using plasmid encoding galectin-3 (Gal-3) led to an improvement in Th2 allergic inflammation. Methods. Using a microarray approach, this study endeavored to evaluate the changes produced by therapeutic Gal-3 delivered by gene therapy in a well-characterized mouse model of chronic airway inflammation. Results were confirmed by real-time RT-PCR, Western blot and immunohistochemical analysis. Results. We identify a set of genes involved in different pathways whose expression is coordinately decreased/increased in mice treated with Gal-3 gene therapy. We report a correlation between Gal-3 treatment and inhibition of SOCS1 and SOCS3 expression in lungs. Conclusion. These results suggest that negative regulation of SOCS1 and 3 following Gal-3 treatment could be a valuable therapeutic approach in allergic disease

Topics: Research Article
Publisher: Hindawi Publishing Corporation
OAI identifier:
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles


    1. (2002). A .B a n e r j e e ,A .S .B a n k s ,M .C .N a w i j n
    2. (2002). A regulatory role for suppressor of cytokine
    3. (2003). A role of suppressor of cytokine signaling 3 (SOCS3/CIS3/SSI3) in CD28-mediated interleukin 2 production,”
    4. (2003). A s t r a n d ,a n dT .P .S p e e d , “A comparison of normalization methods for high density oligonucleotide array data based on variance and bias,”
    5. (2001). Analysis of relative gene expression data using real-time quantitative
    6. (2000). Asthma: from bronchoconstriction to airways inflammation and remodeling,”
    7. (1989). Bronchial biopsies in asthma. An ultrastructural, quantitative study and correlation with hyperreactivity,”
    8. (1998). C´ ardabaetal.,“Galectin-3downregulates IL-5 gene expression on different cell types,”
    9. (1996). Expression monitoring by hybridization to high-density oligonucleotide arrays,”
    10. (2002). Expression of the suppressor of cytokine signaling-5 (SOCS5) negatively regulates IL-4-dependent STAT6 activation and
    11. (2010). Flt3-ligand decreases Th17 cells and SOCS proteins in the lung of house dust mite-sensitized and challenge mice,”
    12. (2007). From genes to functional classes in the study of biological systems,”
    13. (2002). Galectins and their ligands: amplifiers, silencers or tuners of the inflammatory response?”
    14. (2002). Galectins as inflammatory mediators,”
    15. (2005). Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles,”
    16. (2002). Gene therapy with galectin-3 inhibits bronchial obstruction and inflammation in antigen-challenged rats through interleukin-5 gene downregulation,”
    17. (1988). IgEbinding protein. Subcellular location and gene expression in many murine tissues and cells,”
    18. (2006). Inhibition of chronic airway inflammation and remodeling by Galectin-3 gene therapy in a murine model,”
    19. (1995). Quantitative monitoring of gene expression patterns with a complementary
    20. (2004). R.C.Gentleman,V.J.Carey,D.M.Batesetal.,“Bioconductor: open software development for computational biology and bioinformatics,”
    21. (2003). Regulation of cytokine signaling
    22. (2007). SOCS proteins, cytokine signalling and immune regulation,”
    23. (2004). SOCS: role in inflammation, allergy and homeostasis,”
    24. (2006). Suppressor of cytokine signaling 3 (SOCS3)
    25. (2003). Suppressor of cytokine signaling 3 regulatesMediators of Inflammation 9 proliferation and activation of T-helper cells,”
    26. (2007). Suppressor of cytokine signaling in allergic inflammation,”
    27. (2003). Suppressors of cytokine signaling and immunity,”
    28. (2002). Suppressors of cytokine signaling proteins are differentially expressed in Th1 and Th2 cells: implications for Th cell lineage commitment and maintenance,”
    29. (2004). The role of Suppressors of Cytokine Signaling (SOCS) proteins in regulation of the immune response,”
    30. (2005). V a q u e r i z a s ,L .C o n d e ,P .Y a n k i l e v i c he ta l .
    31. (1995). Y.BenjaminiandY.Hochberg,“Controllingthefalsediscovery rate: a practical and powerful approach to multiple testing,”
    32. (2004). Z u b e r i ,D .K .H s u ,O .K a l a y c ie ta l . ,“ C r i t i c a lr o l ef o r galectin-3 in airway inflammation and bronchial hyperresponsiveness in a murine model of asthma,”

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.