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The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds

By Chunyan Zhang, Yun Liu, Kunkun Huang, Wenbing Zeng, Deqing Xu, Qunying Wen and Liguo Yang

Abstract

Two active mutations (A 781 G and A 1575 G) in growth hormone (GH) gene, and their associations with litter size (LS), were investigated in both a high prolificacy (Matou, n = 182) and a low prolificacy breed (Boer, n = 352) by using the PCR-RFLP method. Superovulation experiments were designed in 57 dams, in order to evaluate the effect of different genotypes of the GH gene on superovulation response. Two genotypes (AA and AB, CC and CD) in each mutation were detected in these two goat breeds. Neither BB nor DD homozygous genotypes were observed. The genotypic frequencies of AB and CC were significantly higher than those of AA and CD. In the third parity, Matou dams with AB or CC genotypes had significantly larger litter sizes than those with AA and CD (p < 0.05). On combining the two loci, both Matou and Boer dams with ABCD genotype had the largest litter sizes when compared to the other genotypes (p < 0.05). When undergoing like superovulation treatments, a significantly higher number of corpora lutea and ova, with a lower incidence of ovarian cysts, were harvested in the AB and CC genotypes than in AA and CD. These results show that the two loci of GH gene are highly associated with abundant prolificacy and superovulation response in goat breeds

Topics: Animal Genetics
Publisher: Sociedade Brasileira de Genética
OAI identifier: oai:pubmedcentral.nih.gov:3085373
Provided by: PubMed Central

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Citations

  1. (2009). A miniature condition in Brahman cattle is associated with a single nucleotide mutation within the growth hormone gene.
  2. (2009). AluI polymorphism of the bovine growth hormone (GH) gene, resumption of ovarian cyclicity, milk production and lossofbodyconditionattheonsetoflactationindairycows.
  3. (2009). Association analysis of the INHA gene with litter size in Boer goats.
  4. (2003). Effect of GH and IGF-I treatment on reproduction, growth, and plasma hormone concentrations in domestic nutria (Myocastor coypus).
  5. (2008). Effects of gonadotrophins, growth gormone, and activin A on enzymatically isolated follicle growth, oocyte chromatin organization, and steroid secretion.
  6. (2003). Expression of growth hormone and its transcription factor, Pit-1, in early bovine development. Mol Reprod Dev 64:275-283. KmieM,TermanA,WierzbickiHandZychS(2007)Association of GH gene polymorphism with semen parameters of boars.
  7. (2002). GH as a co-gonadotropin: The relevance of correlative changes in GH secretion and reproductive state.
  8. (2009). GnRHR gene polymorphisms and their effects on reproductive performance in Chinese goats.
  9. (2001). Growth hormone (GH)/GH receptor expression and GH-mediated effects during early bovine embryogenesis.
  10. (1998). Growth hormone and ovarian function. Baillieres Clin Endocrinol Metab 12:331-340. GongJG(2002)Influenceofmetabolichormonesandnutritionon ovarian follicle development in cattle: Practical implications.
  11. (1996). In vitro maturation of bovine oocytes in the presence of growth hormone accelerates nuclear maturation and promotes subsequent embryonic development.
  12. (2003). Interactions between nutrition and ovarian activity in cattle: Physiological, cellular and molecular mechanisms.
  13. (1998). Isolated porcine ovarian follicles as a model for the study of hormone and growth factor action on ovarian secretory activity.
  14. LiangAX,HanLandGengLY(2008)Polymorphismofthe growth hormone gene and its association with growth traits in Boer goat bucks.
  15. (2007). Oestradiol concentration as a predictor of ovarian response in FSH stimulated ewe-lambs.
  16. (2004). Polymorphism analysis of goat growth hormone (GH) gene by PCR-RFLP.
  17. (2003). Polymorphism of growth hormone gene in 12 pig breedsanditsrelationshipwithpiggrowthandcarcasstraits.
  18. (2001). Polymorphisms at the five exons of the growth hormone gene in the algarvia goat: Possible association with milk traits.
  19. (1999). Possible role of growth hormone, IGFs, and IGF binding proteins in the regulation of ovarian function in large farm animals.
  20. (2003). Renaville R and Cravador A
  21. (2008). Reproductive performance of Matou goatundersub-tropicalmonsoonalclimateofCentralChina. Trop Anim Health Prod
  22. (2005). Restricted maximum likelihood estimation of genetic principal components and smoothed covariance matrices.
  23. (2009). Review: Involvementofgrowthhormone(GH)andinsulin-likegrowth factor (IGF) system in ovarian folliculogenesis.
  24. (2007). Single nucleotide polymorphism in growth hormone gene exon-4 and exon-5 using PCR-SSCP in Black Bengal goats - A prolific meat breed of India.
  25. (2009). SNPs detection of growth hormone gene in Gannan Yak population.
  26. (1998). Soom A and de Kruif A
  27. (1998). The promotory effect of growth hormone on the developmental competence of in vitro matured bovine oocytes is due to improved cytoplasmic maturation.
  28. (2008). Transmission ratio distortion at the growth hormone gene (GH1) in bovine preimplantation embryos: An in vitro culture-induced phenomenon? Mol Reprod Dev