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Cell-enclosing gelatin-based microcapsule production for tissue engineering using a microfluidic flow-focusing system

By Shinji Sakai, Sho Ito, Hitomi Inagaki, Keisuke Hirose, Tomohiro Matsuyama, Masahito Taya and Koei Kawakami


Gelatin-based microcapsule production using a microfluidic system and the feasibility of the resultant microcapsules for constructing spherical tissues surrounded by heterogeneous cells were studied. The first cell-encapsulation and subsequent cell-enclosing microparticle encapsulation were achieved using a microfluidic flow-focusing droplet production system. A hollow-core structure of about 150 μm in diameter was developed by incubating the resultant microparticles at 37 °C, which induced thermal melting of the enclosed unmodified gelatin microparticles. Mammalian cells filled the hollow-cores after 4 days of incubation. A cell layer on the cell-enclosing microcapsules was developed by simply suspending the microcapsules in medium containing adherent fibroblast cells. This method may prove useful for the generation of gelatin microcapsules using a microfluidic system for formation of artificial tissue constructs

Topics: Special Topic: Biological Microfluidics in Tissue Engineering and Regenerative Medicine (Guest Editor: Suwan Jayasinghe)
Publisher: American Institute of Physics
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Provided by: PubMed Central
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