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Generation of Viable Male and Female Mice from Two Fathers1

By Jian Min Deng, Kei Satoh, Hongran Wang, Hao Chang, Zhaoping Zhang, M. David Stewart, Austin J. Cooney and Richard R. Behringer


In sexual species, fertilization of oocytes produces individuals with alleles derived from both parents. Here we use pluripotent stem cells derived from somatic cells to combine the haploid genomes from two males to produce viable sons and daughters. Male (XY) mouse induced pluripotent stem cells (Father #1) were used to isolate subclones that had spontaneously lost the Y chromosome to become genetically female (XO). These male-derived XO stem cells were used to generate female chimeras that were bred with genetically distinct males (Father #2), yielding progeny possessing genetic information that was equally derived from both fathers. Thus, functional oocytes can be generated from male somatic cells after reprogramming and spontaneous sex reversal. These findings have novel implications for mammalian reproduction and assisted reproductive technology

Topics: Research Article
Publisher: Society for the Study of Reproduction, Inc.
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Provided by: PubMed Central
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