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Protein solubility and differential proteomic profiling of recombinant Escherichia coli overexpressing double-tagged fusion proteins

By Chung-Hsien Cheng and Wen-Chien Lee
Topics: Research
Publisher: BioMed Central
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Provided by: PubMed Central

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  1. (2007). A: Divergent genetic control of protein solubility and conformational quality in Escherichia coli.
  2. (2006). A: Protein quality in bacterial inclusion bodies. Trends Biotechnol
  3. (2005). A: Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors. Microb Cell Fact
  4. (2001). Adenosine diphosphate sugar pyrophosphatase prevents glycogen biosynthesis in Escherichia coli.
  5. (2005). Advanced genetic strategies for recombinant protein expression in Escherichia coli.
  6. (1992). AM: Two novel heat shock genes encoding proteins produced in response to heterologous protein expression in Escherichia coli.
  7. (2005). Aon JC: Proteomic profiling of Escherichia coli proteins under high cell density fed-batch cultivation with overexpression of phosphogluconolactonase. Biotechnol Prog
  8. (2004). CA: A chaperone network controls the heat shock response
  9. (2003). Comparison of the Escherichia coli proteomes for recombinant human growth hormone producing and nonproducing fermentations. Proteomics
  10. (2005). CP: Homocysteine toxicity in Escherichia coli is caused by a perturbation of branched-chain amino acid biosynthesis.
  11. (2005). Deckwer WD: Proteome analysis of a recombinant Bacillus megaterium strain during heterologous production of a glucosyltransferase. Proteome Sci
  12. (2006). Effects of the presence of
  13. (2003). Engineering Escherichia coli for increased productivity of serine-rich proteins based on proteome profiling. Appl Environ Microbiol
  14. (1999). FR: Genome-wide expression profiling in Escherichia coli K-12. Nucleic Acids Res
  15. (2003). Global metabolic regulation analysis for Escherichia coli K12 based on protein expression by 2-dimensional electrophoresis and enzyme activity measurement. Appl Microbiol Biotechnol
  16. (2001). Goloubinoff P: Review: mechanisms of disaggregation and refolding of stable protein aggregates by molecular chaperones.
  17. (1996). HslV-HslU: A novel ATP-dependent protease complex in Escherichia coli related to the eukaryotic proteasome. Proc Natl Acad Sci USA
  18. (2006). In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat Protoc
  19. (2004). Inclusion bodies: formation and utilisation. Adv Biochem Engin/Biotechnol
  20. (1992). Isopropyl-b-d-thiogalactopyranoside influences the metabolism of Escherichia coli.
  21. (2003). KH: Escherichia coli–a model system that benefits from and contributes to the evolution of proteomics. Biotechnol Bioeng
  22. (2005). KM: Proteomic profiling of reconbinant Escherichia coli in high-cell-density fermentations for improved production of an antibody fragment biopharmaceutical. Appl Environ Microbiol
  23. (2007). Le Chatelier E, Ehrlich SD: Genetic evidence for a link between glycolysis and DNA replication. PLoS ONE
  24. (2000). Liao JC: DNA microarray detection of metabolic responses to protein overproduction in Escherichia coli. Metab Eng
  25. (2003). Mar Carrió M: Protein aggregation in recombinant bacteria: biological role of inclusion bodies. Biotechnol Lett
  26. (2006). Melefors O: The Escherichia coli BarA-UvrY two-component system is a virulence determinant in the urinary tract.
  27. (2000). MJ: A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry. Electrophoresis
  28. (2009). Production of N-acetylD-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteins.
  29. (2009). Proteomic changes in the hypothalamus and retroperitoneal fat from male F344 rats subjected to repeated light-dark shifts. Proteomics
  30. (2007). Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips. Nat Protoc
  31. (1981). R.Fersht A: Alternative pathways for editing non-cognate amino acids by aminoacyl-tRNA synthetases. Nucleic Acids Res
  32. (1999). RG: New fusion protein systems designed to give soluble expression in Escherichia coli. Biotechnol Bioeng
  33. (2004). Roles and applications of small heat shock proteins in the production of recombinant proteins in Escherichia coli. Biotechnol Bioeng
  34. (1989). Sauer RT: Induction of a heat shock-like response by unfolded protein in Escherichia coli: dependence on protein level not protein degradation.
  35. (2000). T: Monitoring of genes that respond to overproduction of an insoluble recombinant protein in Escherichia coli glucose-limited fed-batch fermentations. Biotechnol Bioeng
  36. (2006). The Escherichia coli proteome: past, present, and future prospects. Microbiol Mol Biol Rev