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The Arabidopsis plastid-signalling mutant gun1 (genomes uncoupled1) shows altered sensitivity to sucrose and abscisic acid and alterations in early seedling development

By Amanda Cottage, Ellie K. Mott, Jennie A. Kempster and John C. Gray

Abstract

Developing seedlings of the Arabidopsis gun1 (genomes uncoupled1) mutant, which is defective in retrograde plastid-to-nucleus signalling, show several previously unrecognized mutant phenotypes. gun1 seedlings accumulated less anthocyanin than wild-type seedlings when grown in the presence of 2% (w/v) sucrose, due to lower amounts of transcripts of early anthocyanin biosynthesis genes in gun1. Norflurazon and lincomycin, which induce retrograde signalling, further decreased the anthocyanin content of sucrose-treated seedlings, and altered the temporal pattern of anthocyanin accumulation. Lincomycin treatment altered the spatial pattern of sucrose-induced anthocyanin accumulation, suggesting that plastids provide information for the regulation of anthocyanin biosynthesis in Arabidopsis seedlings. The temporal pattern of accumulation of LHCB1 transcripts differed between wild-type and gun1 seedlings, and gun1 seedlings were more sensitive to sucrose suppression of LHCB1 transcript accumulation than wild-type seedlings. Growth and development of gun1 seedlings was more sensitive to exogenous 2% sucrose than wild-type seedlings and, in the presence of lincomycin, cotyledon expansion was enhanced in gun1 seedlings compared to the wild type. gun1 seedlings were more sensitive than wild-type seedlings to the inhibition of seedling growth and development by abscisic acid. These observations clearly implicate GUN1 and plastid signalling in the regulation of seedling development and anthocyanin biosynthesis, and indicate a complex interplay between sucrose and plastid signalling pathways

Topics: Research Papers
Publisher: Oxford University Press
OAI identifier: oai:pubmedcentral.nih.gov:2921207
Provided by: PubMed Central

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Citations

  1. (1997). A deletion in the PHYD gene of the arabidopsis Wassilewskija ecotype defines a role for phytochrome D in red/far-red light sensing.
  2. (2000). A prediction of the size and evolutionary origin of the proteome of chloroplasts of Arabidopsis. Trends
  3. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures.
  4. (1996). An intracellular signal transduction pathway between the chloroplast and nucleus is involved in de-etiolation.
  5. (2006). Arabidopsis ABA INSENSITIVE4 regulates lipid mobilization in the embryo and reveals repression of seed germination by the endosperm.
  6. (2001). Arabidopsis genomes uncoupled 5 (GUN5) mutant reveals the involvement of Mg-chelatase H subunit in plastid-to-nucleus signal transduction.
  7. (2006). Arabidopsis UPF1 RNA helicase for nonsense-mediated mRNA decay is involved in seed size control and is essential for growth.
  8. (2001). Bleaching herbicide norflurazon inhibits phytoene desaturase by competition with the cofactors.
  9. (1984). Carotenoid-deficient maize seedlings fail to accumulate light-harvesting chlorophyll a/b binding protein (LHCP) mRNA.
  10. (1995). Chloroplast control of nuclear gene expression. In:
  11. (2003). Chloroplast to nucleus communication triggered by accumulation of Mg-protoporphyrinIX.
  12. (1991). Chloroplast transcription is required to express the nuclear genes rbcS and cab. Plastid DNA copy number is regulated independently.
  13. (2006). Chloroplast-mediated regulation of nuclear genes in Arabidopsis thaliana in the absence of light stress.
  14. (2004). Comprehensive identification of Arabidopsis thaliana MYB transcription factors interacting with R/B-like BHLH proteins.
  15. (1994). Control of leaf and chloroplast development by the Arabidopsis gene pale cress.
  16. (2008). Coordination of gene expression between organellar and nuclear genomes.
  17. (2003). Coordination of plastid and nuclear gene expression.
  18. (1979). Cytoplasmic synthesis of plastid polypeptides may be controlled by plastid-synthesized RNA.
  19. (1996). DAG, a gene required for chloroplast differentiation and palisade development in Antirrhinum majus.
  20. (2007). Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling.
  21. (1999). Disruption of specific flavonoid genes enhances the accumulation of flavonoid enzymes and end-products in Arabidopsis seedlings.
  22. (2001). Effects of sugar on vegetative development and floral transition in Arabidopsis.
  23. Energy from the sun: 14th International Congress on Photosynthesis.
  24. (2002). Evolutionary analysis of Arabidopsis, cyanobacterial, and chloroplast genomes reveals plastid phylogeny and thousands of cyanobacterial genes in the nucleus.
  25. (1986). Expression of nuclear genes as affected by treatments acting on the plastids.
  26. (2008). Ferna ´ndez A, Strand A ˚ .
  27. (2001). Growth stage-based phenotypic analysis Interaction of sucrose and plastid signalling pathways | 3783of Arabidopsis: a model for high throughput functional genomics in plants.
  28. (2008). GUN1 (GENOMES UNCOUPLED1) encodes a pentatricopeptide repeat (PPR) protein involved in plastid protein synthesis-responsive retrograde signaling to the nucleus. In:
  29. (2003). GUN4, a regulator of chlorophyll synthesis and intracellular signaling.
  30. (2006). h e lba1 mutation of UPF1 RNA helicase involved in nonsensemediated mRNA decay causes pleiotropic phenotypic changes and altered sugar signalling in Arabidopsis.
  31. (2000). Interactions between hy1 and gun mutants of Arabidopsis, and their implications for plastid/nuclear signalling.
  32. (1988). Light effects on several chloroplast components in norflurazontreated pea leaves.
  33. (1995). Light intensity regulation of cab gene transcription is signaled by the redox state of the plastoquinone pool.
  34. (1998). Metabolic bypass of the tricarboxylic acid cycle during lipid mobilization in germinating oilseeds. Regulation of NAD +-dependent isocitrate dehydrogenase versus fumarase.
  35. (2002). Mobilization of seed storage lipid by Arabidopsis seedlings is retarded in Interaction of sucrose and plastid signalling pathways | 3785the presence of exogenous sugars.
  36. (1997). Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for beta-amylase and on the accumulation of anthocyanin that are inducible by sugars.
  37. (1975). On the prokaryotic nature of red algal chloroplasts.
  38. (1987). Organ-specific and dosage-dependent expression of a leaf/stem specific gene from potato after tagging and transfer into potato and tobacco plants.
  39. (2008). Pentatricopeptide repeat proteins: a socket set for organelle gene expression.
  40. (1988). Photooxidation of plastids inhibits transcription of nuclear encoded genes in rye (Secale cereale).
  41. (1986). Photooxidative destruction of chloroplasts and its consequences for expression of nuclear genes.
  42. (1986). Photosynthesis-associated gene families: differences in response to tissue-specific and environmental factors.
  43. (2000). Photosynthesis, sugars and the regulation of gene expression.
  44. (2001). Plastid redox state and sugars: interactive regulators of nuclear-encoded photosynthetic gene expression.
  45. (2008). Plastid signalling to the nucleus and beyond.
  46. (2009). Plastid signalling to the nucleus: messengers still lost in the mists? Trends in
  47. (2007). Plastid signals remodel light signaling networks and are essential for efficient chloroplast biogenesis in Arabidopsis.
  48. (2009). Plastid signals that affect photomorphogenesis
  49. (1999). Plastid translation is required for the expression of nuclear photosynthesis genes in the dark and in the roots of the pea lip1 mutant.
  50. (2006). Plastid-to-nucleus retrograde signaling.
  51. (1998). Pleiotropic control of glucose and hormone responses by PRL1, a nuclear WD protein, in Arabidopsis.
  52. (1992). Regulation of flavonoid biosynthetic genes in germinating Arabidopsis seedlings.
  53. (2005). Retrograde plastid redox signals in the expression of nuclear genes for chloroplast proteins of Arabidopsis thaliana.
  54. (1980). Secondary plant products.
  55. (1993). Signal transduction mutants of arabidopsis uncouple nuclear CAB and RBCS gene expression from chloroplast development.
  56. (2007). Signals from chloroplasts converge to regulate nuclear gene expression.
  57. (1997). Sucrose control of phytochrome A signalling in Arabidopsis.
  58. (2005). Sucrose-specific induction of anthocyanin biosynthesis
  59. (2006). Sucrose-specific induction of the anthocyanin biosynthetic pathway in Arabidopsis.
  60. (2005). Sugar and ABA responsiveness of a minimal RBCS light-responsive unit is mediated by direct binding of ABI4.
  61. (2003). Sugar and hormone connections.
  62. (2004). Sugar and phytohormone response pathways: navigating a signalling network.
  63. (2006). Sugar sensing and signaling in plants: conserved and novel mechanisms.
  64. Sugar-dependent expression of the CHS-A gene for chalcone synthase from petunia in transgenic Arabidopsis.
  65. (2008). Tetrapyrrole profiling in Arabidopsis seedlings reveals that retrograde plastid nuclear signalling is not due to Mg-protoporphyrin accumulation.
  66. (1998). The Arabidopsis abscisic acid response locus ABI4 encodes an APETALA 2 domain protein.
  67. (2001). The Arabidopsis HY2 gene encodes phytochromobilin synthase, a ferredoxin-dependent biliverdin reductase.
  68. (2001). The Arabidopsis immutans mutation affects plastid differentiation and the morphogenesis of white and green sectors in variegated plants.
  69. (1999). The Arabidopsis photomorphogenic mutant hy1 is deficient in phytochrome chromophore biosynthesis as a result of a mutation in a plastid heme oxygenase.
  70. (1999). The Arabidopsis thaliana HY1 locus, required for phytochrome-chromophore biosynthesis, encodes a protein related to heme oxygenases.
  71. (2005). The arabidopsis transcription factor MYB12 is a flavonol-specific regulator of phenylpropanoid biosynthesis.
  72. (1988). The chloroplast affects the transcription of a nuclear gene family.
  73. (1992). The chloroplast genome.
  74. (1996). The DCL gene of tomato is required for chloroplast development and palisade cell morphogenesis in leaves.
  75. (1986). The implication of a plastid-derived factor in the transcriptional control of nuclear genes encoding the light-harvesting chlorophyll a/b protein.
  76. (2004). The nuclear genes Lhcb and HEMA1 are differentially sensitive to plastid signals and suggest distinct roles for the GUN1 and GUN5 plastid-signalling pathways during de-etiolation.
  77. (1994). The role of plastids in the expression of nuclear genes for thylakoid proteins studied with chimeric b-glucuronidase gene fusions.
  78. (1988). Transcription of two photosynthesisassociated nuclear gene families correlates with the presence of chloroplasts in leaves of the variegated tomato ghost mutant.