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Multiphoton microscopy with near infrared contrast agents

By Siavash Yazdanfar, Chulmin Joo, Chun Zhan, Mikhail Y. Berezin, Walter J. Akers and Samuel Achilefu

Abstract

While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications

Topics: JBO Letters
Publisher: Society of Photo-Optical Instrumentation Engineers
OAI identifier: oai:pubmedcentral.nih.gov:2881927
Provided by: PubMed Central
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