龙须菜试剂级R-型藻红蛋白的高效分离纯化方法
Abstract
本发明属于海洋天然活性物质制备技术，具体的说是一种龙须菜试剂级R-型藻红蛋白的高效分离纯化方法。将粉碎的原料大型经济海洋红藻龙须菜加入其10-30体积的PBS缓冲液中浸泡，浸泡后以9000rpm/min-15000rpm/min离心20-30min，收集上清液进行浓缩；浓缩液经硫酸铵进行分级盐析，收集沉淀并用PBS缓冲液溶解，溶解后进行透析24--48小时；所得透析液经强阴离子Source-15Q层析柱纯化，以含NaCl的PBS缓冲液作为洗脱液，流速0.5ml/min,收集第360min-510min的洗脱液，即为纯度大于5.0的R-型藻红蛋白溶液。采用本发明方法得到的蛋白回收率高，纯化效果好，易于放大，上样量大等特点实现了对藻红蛋白高效分离纯化Similar works
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Last time updated on 04/07/2017
This paper was published in Institutional Repository of Yantai Institute of Coastal Zone Research, CAS.
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