B2 receptor blockage prevents Aβ-induced cognitive impairment by neuroinflammation inhibition

Abstract

Background and purpose: Aβ-induced neuronal toxicity and memory loss is thought to be dependent on neuroinflammation, an important event in Alzheimer's disease (AD). Previously, we demonstrated that the blockage of the kinin B receptor (BR) protects against the memory deficits induced by amyloid β (Aβ) peptide in mice. In this study, we aimed to investigate the role of BR on Aβ-induced neuroinflammation in mice and the beneficial effects of BR blockage in synapses alterations. Experimental approach: The selective kinin BR antagonist HOE 140 (50pmol/site) was given by intracerebroventricular (i.c.v.) route to male Swiss mice 2h prior the i.c.v. injection of Aβ (400pmol/site) peptide. Animals were sacrificed, at specific time points after Aβ injection (6h, 1 day or 8 days), and the brain was collected in order to perform immunohistochemical analysis. Different groups of animals were submitted to behavioral cognition tests on day 14 after Aβ administration. Key results: In this study, we report that the pre-treatment with the selective kinin BR antagonist HOE 140 significantly inhibited Aβ-induced neuroinflammation in mice. BR antagonism reduced microglial activation and the levels of pro-inflammatory proteins, including COX-2, iNOS and nNOS. Notably, these phenomena were accompanied by an inhibition of MAPKs (JNK and p38) and transcription factors (c-Jun and p65/NF-κB) activation. Finally, the anti-inflammatory effects of BR antagonism provided significant protection against Aβ-induced synaptic loss and cognitive impairment in mice. Conclusions and implications: Collectively, these results suggest that BR activation may play a critical role in Aβ-induced neuroinflammation, one of the most important contributors to AD progression, and its blockage can provide synapses protection