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Dengue virus-induced cytotoxin releases nitrite by spleen cells

By RUMA MUKERJEE, ANITA MISRA and U.C. CHATURVEDI

Abstract

Dengue type-2 virus (DV) infection in mice induces T cells to produce a cytokine, the cytotoxic factor (CF), which induces H2-A positive macrophages to produce another cytokine, cyotoxin (CF2), which amplifies its cytotoxic effects on target cells. The present study was undertaken to investigate the production of nitrite (NO−2) by the spleen cells of mice in vitro and in vivo following inoculation of CF2. Maximum NO−2 production occurred at 1 hour after inoculation of 100 μg CF2. Pretreatment of CF2 with anti-CF2-antisera (CF2-As) inhibited the production of NO−2. Pretreatment of the spleen cells with NG-monomethyl- l-arginine (NMA) or with arginase inhibited NO−2 production. The NO−2 production was diminished in a dose dependent manner by treatment of spleen cells with the Ca2+ channel blocking drug, nifedipine and Zn2+ as ZnSO4. The findings of the present study thus demonstrate that CF2 induces production of NO−2 in the spleen cells in a CA2+-dependent manner which may be a mechanism of target cell killing

Topics: Original Articles
Publisher: Blackwell Publishing
OAI identifier: oai:pubmedcentral.nih.gov:2691624
Provided by: PubMed Central
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