Skip to main content
Article thumbnail
Location of Repository

NFI is an Essential Positive Transcription Factor for Human Papillomavirus Type 16 Early Gene Expression

By Amy Baldwin, Melissa K Hypes, Lucia Pirisi and Kim E Creek

Abstract

Human papillomavirus type 16 (HPV16) is the primary etiologic agent for greater than 50% of all cervical carcinomas. Expression of the HPV16 E6 and E7 oncoproteins is under control of the upstream regulatory region (URR), which contains a myriad of transcription factor binding sites, including 7 half sites for NFI. These NFI binding sites were used as probes in electrophoretic mobility shift assays (EMSAs), and mutational analysis of individual and multiple NFI binding sites was performed in order to demonstrate the relative importance of particular NFI sites to URR activity. By using 5 NFI half sites as an enhancer, we were able to detect a 4-fold increase in URR activity. Our results define the role and relative contribution of NFI binding sites to the basal activity of the HPV16 promoter, and demonstrate that NFI binding sites can act independently to enhance HPV16 URR activity in immortalized keratinocytes

Topics: Article
Publisher: Bentham Science Publishers Ltd.
OAI identifier: oai:pubmedcentral.nih.gov:2675546
Provided by: PubMed Central
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://www.pubmedcentral.nih.g... (external link)
  • Suggested articles

    Citations

    1. (1996). Papillomavirus infections--a major cause of human cancers.
    2. (2000). Papillomaviruses causing cancer: evasion from hostcell control in early events in carcinogenesis.

    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.