Spectral Unmixing of Flavin Autofluorescence Components in Cardiac Myocytes

Chorvat, D.; Kirchnerova, J.; Cagalinec, M.; Smolka, J.; Mateasik, A.; Chorvatova, A.

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doi:10.1529/biophysj.105.073866

Spectral Unmixing of Flavin Autofluorescence Components in Cardiac Myocytes

Authors: D. Chorvat
J. Kirchnerova
M. Cagalinec
J. Smolka
A. Mateasik
A. Chorvatova
Publication date: 31 December 2005
Publisher: The Biophysical Society. Published by Elsevier Inc.
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Abstract

AbstractWe applied linear unmixing approach to reveal individual components of intrinsic flavin fluorescence signal recorded in living cardiac cells by spectrally resolved confocal microscopy. Responses of whole-cell autofluorescence to modulators of cell metabolism and respiration were used as a tool of separation of its components; their spectral profiles, estimated by principal component analysis, correspond to free FAD and FAD bound to different enzymes of electron transport chain

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doi:10.1529/biophysj.105.07386...

Last time updated on 06/05/2017

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