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Inhibitory Effects of Partially Decomposed Alginate on Production of Glucan and Organic Acid by Streptococcus sobrinus 6715

By Michiru Hashiguchi-Ishiguro, Sadako Nakamura and Tsuneyuki Oku

Abstract

Our previous study has already clarified that partially decomposed alginate (Alg53) by Vibrio alginolyticus SUN53 has a competitive inhibitory effect on sucrase. The objective of this study is to evaluate the influence of Alg53 on the production of glucan from sucrose by glucosyltransferase and acid from glucose by Streptococcus sobrinus 6715. Glucosyltransferase was prepared from cultural medium of S. sobrinus using ultrafiltration and hydroxyapatite chromatography. In order to examine the inhibitory effect of Alg53 for production of glucan by GTase, partially purified GTase, sucrose and Alg53 solution were incubated at 37°C. The influence of Alg53 on the production of acid from glucose was evaluated by a degree of pH decline during the incubation for 60 min. The original Alg53 solution markedly inhibited to 21% of the synthesis of water-insoluble glucan from sucrose and that of 10-fold diluted of Alg53 solution was 23%. However, the production of water-soluble glucan from sucrose by GTase was hardly affected by Alg53. Furthermore, Alg53 suppressed dose-dependently pH decline by organic acid converted from glucose. These results suggest that Alg53 is expected as a functional food material which prevents or reduces dental caries

Topics: Original Article
Publisher: the Society for Free Radical Research Japan
OAI identifier: oai:pubmedcentral.nih.gov:2675025
Provided by: PubMed Central
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    Citations

    1. (1992). Alginate lyase from Vibrio alginolyticus ATCC 17749.
    2. (2000). Alginate lyase: Review of major sources and enzyme characteristics, structure-function analysis, biological roles, and applications.
    3. (2003). Anti-cariogenic properties of a water-soluble extract from Cacao.
    4. (1995). Characterization of glucosyltransferaseB, GtfC, and GtfD in solution and on the surface of hydroxyapatite.
    5. (2002). Cloning and nucleotide sequence analysis of the Streptococcus sobrinus gtfU gene that produces a highly branched water-soluble glucan.
    6. (1993). Close association between Streptococcus sobrinus in the saliva of young children and smooth-surface caries increment.
    7. (1956). Colorimetric method for determination of sugars and related substances.
    8. (1991). Differences in cariogenicity between fresh isolates of Streptococcus sobrinus and Streptococcus mutans.
    9. (1985). Enamel microhardness change and plaque pH measurements in an intra-oral model in humans.
    10. (2005). In vitro and in vivo effects of isolated fractions of Brazilian propolis on caries development.
    11. (1983). Inhibition by acarbose, nojirimycin and 1-deoxynojirimycin of glucosyltransferase produced by oral streptococci.
    12. (2008). Inhibitory effect of depolymelyzed sodium alginate by Vibrio alginolyticus SUN53 on intestinal brush border membrane disaccharidase in rat.
    13. (1993). Inhibitory effect of oolong tea polyphenols on glucosyltransferases of mutansstreptococci.
    14. (2000). Inhibitory effects of apple polyphenols and related compounds on cariogenic factors of mutans streptococci.
    15. (2007). Japanese society for dental health: Statistics of oral health
    16. (1987). Mutans streptococci, lactobacilli and caries prevalence in 11- and 12-year old Icelandic children. Community Dent.
    17. (1951). Protein determination with the Folin phenol reagent.
    18. (1985). Purification and characterization of glucosyltransferases from Streptococcus mutans 6715.
    19. (1962). Recent advance in dental caries research.
    20. (1984). Screening of sugars inhibitory against sucrose-dependent synthesis and adherence of insoluble glucan and acid production by Streptococcus mutans.
    21. (1995). Species constitution of mutans streptococci isolated from cariessusceptible and caries-free students.
    22. (2000). Studies concerning the glucosyltransferase of Streptococcus sanguis.
    23. (1967). Studies on the sequence of uronic acid residues in alginic acid.
    24. (1988). The caries inhibitory effects of GOS-sugar in vitro and in rat experiments.
    25. (2000). The pH of dental plaque in its relation to early enamel caries and dental plaque flora in humans.

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